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dc.creatorLuquin, E. (Esther)-
dc.creatorPerez-Lorenzo, E. (Eva)-
dc.creatorAymerich-Soler, M. S. (María S.)-
dc.creatorMengual, E. (Elisa)-
dc.date.accessioned2010-09-27T11:30:40Z-
dc.date.available2010-09-27T11:30:40Z-
dc.date.issued2009-12-16-
dc.identifier.citationLuquin E, Perez-Lorenzo E, Aymerich MS, Mengual E. Two-color fluorescence labeling in acrolein-fixed brain tissue. J Histochem Cytochem 2010 Apr;58(4):359-368.es_ES
dc.identifier.issn0022-1554-
dc.identifier.urihttp://hdl.handle.net/10171/13044-
dc.description.abstractSUMMARY Acrolein is a potent fixative that provides both excellent preservation of ultrastructural morphology and retention of antigenicity, thus it is frequently used for immunocytochemical detection of antigens at the electron microscopic level. However, acrolein is not commonly used for fluorescence microscopy because of concerns about possible autofluorescence and destruction of the luminosity of fluorescent dyes. Here we describe a simple protocol that allows fine visualization of two fluorescent markers in 40-mm sections from acroleinperfused rat brain. Autofluorescence was removed by pretreatment with 1% sodium borohydride for 30 min, and subsequent incubation in a 50% ethanol solution containing 0.3% hydrogen peroxide enhanced fluorescence labeling. Thus, fluorescence labeling can be used for high-quality detection of markers in tissue perfused with acrolein. Furthermore, adjacent acrolein-fixed sections from a single experiment can be processed to produce high-quality results for electron microscopy or fluorescence labeling.es_ES
dc.language.isoenges_ES
dc.publisherHistochemical Societyes_ES
dc.rightsinfo:eu-repo/semantics/closedAccess-
dc.subjectsodium borohydridees_ES
dc.subjectendogenous peroxidasees_ES
dc.subjectinactivationes_ES
dc.subjectautofluorescencees_ES
dc.subjectethanoles_ES
dc.subjectenhanced penetrationes_ES
dc.subjecthydrogen peroxidees_ES
dc.subjectaldehyde fixationes_ES
dc.titleTwo-color fluorescence labeling in acrolein-fixed brain tissuees_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1369/jhc.2009.954495es_ES

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