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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Terapia celular > DA - CIMA - Oncología - Terapia celular - Artículos de Revista >

Induction of Nod2 in Myelomonocytic and Intestinal Epithelial Cells via Nuclear Factor-kB Activation
Autor(es) : Gutierrez, O. (Olga)
Pipaon, C. (Carlos)
Inoharas, N. (N.)
Fontalba, A. (A.)
Ogura, Y. (Yasunori)
Prosper, F. (Felipe)
Nuñez, G. (Gabriel)
Fernandez-Luna, J.L. (J.L.)
Palabras clave : Materias Investigacion::Ciencias de la Salud
Fecha incorporación: 2002
Editorial : JBC Papers in Press
ISSN: 0021-9258
Cita: Gutiérrez, O., Pipaon, C., Inoharas, N., Fontalba, A., Oguras, Y., Prósper, F. et al. J. biol. chem. 2002, 277(44): 41701–41705
Resumen
Nod2, a member of the Apaf1/Nod protein family, confers responsiveness to bacterial products and activates NF-kB, a ranscription factor that plays a central role in innate immunity. Recently, genetic variation in Nod2 has been associated with susceptibility to Crohn’s disease. Here, we report that expression of Nod2 is induced upon differentiation of CD34+ hematopoietic progenitor cells into granulocyte or monocyte/macrophages. In peripheral blood cells, the highest levels of Nod2 were observed in CD14+ (monocytes), CD15+ (granulocytes), and CD40+/CD86+ (dendritic cells) cell populations. Notably, stimulation of myeloblastic and epithelial cells with bacterial lipopolysaccharide or TNF resulted in up-regulation of Nod2. A search for consensus sites within the Nod2 promoter revealed a NF-kB binding element that was required for transcriptional activity in response to TNF . Moreover, ectopic expression of p65 induced transactivation, whereas that of dominant-negative I B blocked the transcriptional activity of the Nod2 promoter. Upon stimulation with TNF or lipopolysaccharide, both p50 and p65 subunits of NF-kB were bound to the Nod2 promoter. Thus, Nod2 expression is enhanced by proinflammatory cytokines and bacterial components via NF-kB, a mechanism that may contribute to the amplification of the innate immune response and susceptibility to inflammatory disease.
Enlace permanente: http://hdl.handle.net/10171/17139
Aparece en las colecciones: DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista

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Fichero:  2002_JBC_Gutierrez_Induction of Nod2 in myelomonocytic and intestinal epithelial cells via NF-kB activation.pdf
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