Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Roman-Gomez, J. (José) | |
dc.creator | Jimenez-Velasco, A. (A.) | |
dc.creator | Aguirre-Ena, X. (Xabier) | |
dc.creator | Castillejo, J.A. (J.A.) | |
dc.creator | Navarro, G. (Germán) | |
dc.creator | San-Jose-Eneriz, E. (Edurne) | |
dc.creator | Garate, L. (Leire) | |
dc.creator | Cordeu, L. (Lucía) | |
dc.creator | Cervantes, F. (F.) | |
dc.creator | Prosper-Cardoso, F. (Felipe) | |
dc.creator | Heiniger, A. (A.) | |
dc.creator | Torres, A. (Antonio) | |
dc.date.accessioned | 2011-04-13T14:04:35Z | - |
dc.date.available | 2011-04-13T14:04:35Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Román-Gómez, J., Jiménez-Velasco, A., Agirre, X., Castillejo, J. A. et al. Leukemia Research 2007; 31: 1521-1528 | es_ES |
dc.identifier.issn | 0145-2126 | - |
dc.identifier.uri | https://hdl.handle.net/10171/17701 | - |
dc.description.abstract | Tumor associated antigens (TAA) provide attractive targets for cancer-specific immunotherapy. PRAME is a TAA gene up-regulated in advanced phases of chronic myeloid leukemia (CML). To date, molecular mechanisms for the expression of PRAME have never been studied. We found that some Ph’-positive cell lines did not express PRAME. The expression of PRAMEwas restored in these cell lines by treatment with 5 -aza-2 -deoxycytidine, suggesting that the expression of PRAME is mainly suppressed by hypermethylation. Bisulfite sequencing analysis of the CpG sites of the PRAME exon 2 in these cancer cell lines revealed a close relationship between the methylation status of the PRAME gene and its expression. A methylation-specific PCR analysis demonstrated that hypomethylation of PRAME was significantly more frequent in CML blast crisis (70%) than in chronic phase (36%) (P = 0.01) and was correlated with high expression levels of PRAME transcripts (P < 0.0001). These results suggest that hypomethylation of PRAME up-regulates its expression in CML and might play a significant role in the progression of the disease. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | PRAME | es_ES |
dc.subject | Hypomethylation | es_ES |
dc.subject | CML | es_ES |
dc.title | Epigenetic regulation of PRAME gene in chronic myeloid leukemia | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.sciencedirect.com/science/journal/01452126 | es_ES |
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