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|DNA microarray analysis of genes differentially expressed in diet-induced (cafeteria) obese rats.|
|Authors: ||Lopez, I.P. (Iciar P.)|
Marti, A. (Amelia)
Milagro, F.I. (Fermín I.)
Zulet, M.A. (María Ángeles)
Moreno-Aliaga, M.J. (María Jesús)
Martinez, J.A. (José Alfredo)
Miguel, C. (Carlos) de
|Issue Date: ||2003|
|Publisher: ||North American Association for the Study of Obesity|
|Publisher version: ||http://www.nature.com/oby/journal/v11/n2/full/oby200330a.html|
|Citation: ||Lopez IP, Marti A, Milagro FI, Zulet Md Mde L, Moreno-Aliaga MJ, Martinez JA, et al. DNA microarray analysis of genes differentially expressed in diet-induced (cafeteria) obese rats. Obes Res 2003 Feb;11(2):188-194.|
Objective: To better understand the molecular basis of dietary obesity, we examined adipose tissue genes differentially expressed in an obesity model using DNA microarray analysis.
Research Methods and Procedures: We assessed the expression level of over 12,500 transcripts in epididymal fat pads from (cafeteria) obese and control rats with the aid of the array technology.
Results: Cafeteria (obese) rats weighed 50% more and had 2.5-fold higher levels of epididymal fat and elevated levels of circulating leptin. Adipose genes differentially expressed in obese and control rats were categorized into five groups: macronutrient metabolism, transcription factors, hormone receptor and signal transduction, redox and stress proteins, and cellular cytoskeleton. Interestingly, the expression levels of a number of genes involved in lipid metabolism such as glycerol-3-phosphate dehydrogenase, stearoyl coenzyme A desaturase, together with the transcription factors implicated in adipocyte differentiation (CAAT/enhancer binding protein-alpha and peroxisome proliferator-activated receptor-gamma), were significantly increased in obese animals compared with control. The most up-regulated transcripts were the ob (49.2-fold change) and the fatty acid-binding protein genes (15.7- fold change). In contrast, genes related to redox and stress protein were generally down-regulated in obese animals compared with the control.
Discussion: Our study showed that in diet-induced obesity, the expression levels of some important genes implicated in lipid metabolism were up-regulated, whereas those related to redox and stress protein were down-regulated in obese animals compared with control. This pattern of gene expression may occur in human obesity cases after high-fat intake.
|Permanent link: ||http://hdl.handle.net/10171/17849|
|Appears in Collections:||DA - Farmacia - CAFT - Artículos de revista|
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