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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Terapia celular > DA - CIMA - Oncología - Terapia celular - Artículos de Revista >

Epigenetic regulation of microRNA expression in colorectal cancer
Autor(es) : Bandres, E. (Eva)
Aguirre, X. (Xavier)
Bitarte, N. (Nerea)
Ramirez, N. (Natalia)
Zarate, R. (Ruth)
Roman-Gomez, J. (José)
Prosper, F. (Felipe)
Garcia-Foncillas, J. (Jesús)
Palabras clave : Materias Investigacion::Ciencias de la Salud::Oncología
Fecha incorporación: 2009
Editorial : Wiley-Blackwell
Versión del editor: http://dx.doi.org/10.1002/ijc.24638
ISSN: 0020-7136
Cita: Bandrés, E., Agirre, X., Bitarte, N., Ramírez, N. et al. Epigenetic regulation of microRNA expression in colorectal cancer. Int J Cancer. 2009; 125(11): 2737-43.
Resumen
In the last years, microRNAs (miRNA) have emerged as new molecular players involved in carcinogenesis. Deregulation of miRNAs expression has been shown in different human cancer but the molecular mechanism underlying the alteration of miRNA expression is unknown. To identify tumor-supressor miRNAs silenced through aberrant epigenetic events in colorectal cancer (CRC), we used a sequential approach. We first identified 5 miRNAs down-regulated in colorectal cancer patient samples and located around/on a CpG island. Treatment with a DNA methyltransferase inhibitor and a HDAC inhibitor restored expression of 3 of the 5 microRNAs (hsa-miR-9, hsa-miR-129 and hsa-miR-137) in 3 CRC cell lines. Expression of hsa-miR-9 was inversely correlated with methylation of their promoter regions as measure by MSP and bisulphate sequencing. Further, methylation of the hsa-miR-9-1, hsa-miR-129-2 and hsa-miR- 137 CpG islands were frequently observed in CRC cell lines and in primary CRC tumors, but not in normal colonic mucosa. Finally, methylation of hsa-miR-9-1 was associated with the presence of lymph node metastasis. In summary, our results aid in the understanding of miRNA gene regulation showing that aberrant DNA methylation and histone modifications work together to induce silencing of miRNAs in CRC.
Enlace permanente: http://hdl.handle.net/10171/17938
Aparece en las colecciones: DA - CIMA - Oncología - Síndromes mieloproliferativos - Artículos de Revista
DA - CIMA - Oncología - Terapia celular - Artículos de Revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Medicina - Hematología - Artículos de revista

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Fichero:  2009_IJC_Bandres_Epigenetic regulation of microRNA expression in colorectal cancer.pdf
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