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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Biomarcadores > DA - CIMA - Oncología - Biomarcadores - Artículos de Revista >

Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant alpha CP-4a, is underexpressed in lung cancer
Autor(es) : Pio, R. (Rubén)
Zudaire, I. (Isabel)
Pino, I. (Irene)
Castaño, Z. (Z.)
Zabalegui, N. (Natalia)
Vicent, S. (Silvestre)
Garcia-Amigot, F. (Fermín)
Odero, M.D. (Maria Dolores)
Lozano, M.D. (María Dolores)
Garcia-Foncillas, J. (Jesús)
Calasanz-Abinzano, M.J. (Maria Jose)
Montuenga, L.M. (Luis M.)
Palabras clave : Lung Neoplasms/metabolism
Lung Neoplasms/pathology
RNA-Binding Proteins/biosynthesis
RNA-Binding Proteins/genetics
Fecha incorporación: 2004
Editorial : American Association for Cancer Research
Versión del editor: http://cancerres.aacrjournals.org/content/64/12/4171
ISSN: 1538-7445
Cita: Pio R, Zudaire I, Pino I, Castano Z, Zabalegui N, Vicent S, et al. Alpha CP-4, encoded by a putative tumor suppressor gene at 3p21, but not its alternative splice variant alpha CP-4a, is underexpressed in lung cancer. Cancer Res 2004 Jun 15;64(12):4171-4179.
Resumen
alpha CP-4 is an RNA-binding protein coded by PCBP4, a gene mapped to 3p21, a common deleted region in lung cancer. In this study we characterized the expression of alpha CP-4 and alpha CP-4a, an alternatively spliced variant of alpha CP-4, in lung cancer cell lines and non-small cell lung cancer (NSCLC) samples from early stage lung cancer patients. In NSCLC biopsies, an immunocytochemical analysis showed cytoplasmic expression of alpha CP-4 and alpha CP-4a in normal lung bronchiolar epithelium. In contrast, alpha CP-4 immunoreactivity was not found in 47% adenocarcinomas and 83% squamous cell carcinomas, whereas all of the tumors expressed alpha CP-4a. Besides, lack of alpha CP-4 expression was associated with high proliferation of the tumor (determined by Ki67 expression). By fluorescence in situ hybridization, >30% of NSCLC cell lines and tumors showed allelic losses at PCBP4, correlating with the absence of the protein. On the other hand, no mutations in the coding region of the gene were found in any of the 24 cell lines analyzed. By Northern blotting and real-time reverse transcription-PCR, we detected the expression of alpha CP-4 and alpha CP-4a messages in NSCLC and small cell lung cancer cell lines. Our data demonstrate an abnormal expression of alpha CP-4 in lung cancer, possibly associated with an altered processing of the alpha CP-4 mRNA leading to a predominant expression of alpha CP-4a. This may be considered as an example of alternative splicing involved in tumor suppressor gene inactivation. Finally, induction of alpha CP-4 expression reduced cell growth, in agreement with its proposed role as a tumor suppressor, and suggesting an association of this RNA-binding protein with lung carcinogenesis.
Enlace permanente: http://hdl.handle.net/10171/18810
Aparece en las colecciones: DA - Ciencias - HAP - Artículos de revista
DA - CIMA - Oncología - Biomarcadores - Artículos de Revista
DA - CIMA - Oncología - Genética - Artículos de revista
DA - Ciencias - Genética - Artículos de revista

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