Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Lanciego, J.L. (José Luis) | |
dc.creator | Luquin, M.R. (María Rosario) | |
dc.creator | Guillen, J. (Javier) | |
dc.creator | Gimenez-Amaya, J.M. (José Manuel) | |
dc.date.accessioned | 2011-11-16T12:36:00Z | - |
dc.date.available | 2011-11-16T12:36:00Z | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | Lanciego JL, Luquin MR, Guillen J, Gimenez-Amaya JM. Multiple neuroanatomical tracing in primates. Brain Res Brain Res Protoc 1998 Jun;2(4):323-332. | es_ES |
dc.identifier.issn | 1385-299X | - |
dc.identifier.uri | https://hdl.handle.net/10171/19836 | - |
dc.description.abstract | The present report deals with a multiple tract-tracing procedure in non-human primates enabling the simultaneous visualization of retrogradely transported Fluoro-Gold (FG) and cholera toxin B subunit (CTB) in combination with anterogradely transported biotinylated dextran amine (BDA). Two issues have played key roles on the achievement of this reliable procedure: first, the recent development of a commercial antiserum against FG that allows us to convert the original fluorescent signal of this dye in a permanent precipitate via standard peroxidase-anti-peroxidase methods; second, the introduction of the novel peroxidase substrate Vector(R) VIP (V-VIP), resulting in a purple precipitate. The combination of these neuroanatomical tracers in one and the same histological section opens a possibility for the permanent visualization of the convergence of inputs from a particular brain area onto identified, two different subsets of projection cells of another area. Furthermore, this combination of three tracers emerges as a powerful technical tool for obtaining broad amounts of complementary data regarding the monkey brain connectivity, thus significantly reducing the number of animals needed to complete a particular study. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Axonal tracing | es_ES |
dc.subject | Fluoro-Gold | es_ES |
dc.subject | Cholera toxin | es_ES |
dc.subject | BDA | es_ES |
dc.subject | HRP | es_ES |
dc.subject | V-VIP | es_ES |
dc.subject | Peroxidase | es_ES |
dc.subject | Neuroanatomy | es_ES |
dc.title | Multiple neuroanatomical tracing in primates | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S1385299X98000075 | es_ES |
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