Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Lanciego, J.L. (José Luis) | |
dc.creator | Wouterlood, F.G. (Floris G.) | |
dc.creator | Erro-Aguirre, M.E (María Elena) | |
dc.creator | Gimenez-Amaya, J.M. (José Manuel) | |
dc.date.accessioned | 2011-11-17T12:26:18Z | - |
dc.date.available | 2011-11-17T12:26:18Z | - |
dc.date.issued | 1998 | - |
dc.identifier.citation | Lanciego JL, Wouterlood FG, Erro E, Gimenez-Amaya JM. Multiple axonal tracing: simultaneous detection of three tracers in the same section. Histochem Cell Biol 1998 Nov;110(5):509-515. | es_ES |
dc.identifier.issn | 1432-119X | - |
dc.identifier.uri | https://hdl.handle.net/10171/19865 | - |
dc.description.abstract | Multiple neuroanatomical tract-tracing methods are important tools for elucidating the connectivity between different populations of neurons. Evaluation of the question as to whether two specific fiber inputs converge on a particular, identified population of projection neurons requires the application of a triple-staining procedure that allows the unequivocal detection of three markers in a single section. The present report deals with a combination of tracing methods using anterogradely transported Phaseolus vulgaris leucoagglutinin and biotinylated dextran amine in conjunction with retrogradely transported Fluoro-Gold. These tracers were simultaneously detected according to a three-color paradigm, which includes the use of three different peroxidase substrates (nickel-enhanced diaminobenzidine, diaminobenzidine, and Vector VIP), thus resulting in three distinct precipitates: black, brown, and purple. We illustrate this method by showing convergence of projections arising from neurons located in two separate basal ganglia-related nuclei onto identified thalamostriatal projection neurons. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Springer Verlag | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Tract-tracing | es_ES |
dc.subject | PHA-L | es_ES |
dc.subject | BDA | es_ES |
dc.subject | Fluoro-Gold | es_ES |
dc.subject | V-VIP | es_ES |
dc.subject | Peroxidase | es_ES |
dc.title | Multiple axonal tracing: simultaneous detection of three tracers in the same section | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.springerlink.com/content/q5l4acg4bcquqt4l/ | es_ES |
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