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|The loop diuretic torasemide interferes with endothelin-1 actions in the aorta of hypertensive rats|
|Authors: ||Fortuño, A. (Ana)|
Muñiz, P. (Paula)
Zalba, G. (Guillermo)
Fortuño, M.A. (María Antonia)
Diez, J. (Javier)
|Issue Date: ||2001|
|Publisher: ||Oxford University Press|
|Publisher version: ||http://dx.doi.org/10.1093/ndt/16.suppl_1.18|
|Citation: ||Fortuño A, Muñiz P, Zalba G, Fortuño MA, Diez J. The loop diuretic torasemide interferes with endothelin-1 actions in the aorta of hypertensive rats. Nephrol Dial Transplant 2001;16 Suppl 1:18-21.|
BACKGROUND: The direct effects of torasemide and furosemide on vasoconstriction and increases in intracellular free calcium concentration ([Ca(2+)]i) induced by endothelin-1 (ET-1) were investigated in the aorta of spontaneously hypertensive rats (SHR).
METHODS: Vascular responses were assessed in endothelium-denuded aortic rings using an organ bath system. Changes of [Ca(2+)]i in cultured vascular smooth muscle cells (VSMCs) were assessed using fura-2 methodology.
RESULTS: ET-1-induced vasoconstriction was reduced in a dose-dependent way by torasemide and furosemide (IC(50) values: 4.3+/-1.4x10(-5) and 9.8+/-5.6 x10(-5) M, respectively). The ET-1-induced biphasic [Ca(2+)]i increase was blocked by torasemide (IC(50)=2.0+/-0.2x10(-8) and 2.7+/-0.6x10(-6) M, respectively). Furosemide inhibited the initial rise in [Ca(2+)]i induced by ET-1, with no effect on the second rise. The specific chloride (Cl(-)) channel blocker diphenylamine-2-carboxylate inhibits both ET-1-induced responses in VSMCs (IC(50)=8.0+/-0.3x10(-9) and 2.5+/-0.7x10(-7) M, respectively).
CONCLUSIONS: These results suggest that the ability of loop diuretics to interfere with the vascular actions of ET-1 may involve different molecular mechanisms. The ability of torasemide to block the vasoconstrictive action of ET-1 could include an inhibitory action on Cl(-) channels.
|Permanent link: ||http://hdl.handle.net/10171/19977|
|Appears in Collections:||DA - CIMA - Servicios de apoyo - Biobanco - Artículos de revista|
DA - CIMA - Cardiovasculares - Enfermedad vascular - Artículos de Revista
DA - CIMA - Cardiovasculares - Cardiopatía hipertensiva - Artículos de Revista
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