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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Ciencias Cardiovasculares > Cardiopatía hipertensiva > DA - CIMA - Cardiovasculares - Cardiopatía hipertensiva - Artículos de Revista >

The loop diuretic torasemide interferes with endothelin-1 actions in the aorta of hypertensive rats
Authors: Fortuño, A. (Ana)
Muñiz, P. (Paula)
Zalba, G. (Guillermo)
Fortuño, M.A. (María Antonia)
Diez, J. (Javier)
Keywords: Endothelin-1
Issue Date: 2001
Publisher: Oxford University Press
Publisher version:
ISSN: 0931-0509
Citation: Fortuño A, Muñiz P, Zalba G, Fortuño MA, Diez J. The loop diuretic torasemide interferes with endothelin-1 actions in the aorta of hypertensive rats. Nephrol Dial Transplant 2001;16 Suppl 1:18-21.
BACKGROUND: The direct effects of torasemide and furosemide on vasoconstriction and increases in intracellular free calcium concentration ([Ca(2+)]i) induced by endothelin-1 (ET-1) were investigated in the aorta of spontaneously hypertensive rats (SHR). METHODS: Vascular responses were assessed in endothelium-denuded aortic rings using an organ bath system. Changes of [Ca(2+)]i in cultured vascular smooth muscle cells (VSMCs) were assessed using fura-2 methodology. RESULTS: ET-1-induced vasoconstriction was reduced in a dose-dependent way by torasemide and furosemide (IC(50) values: 4.3+/-1.4x10(-5) and 9.8+/-5.6 x10(-5) M, respectively). The ET-1-induced biphasic [Ca(2+)]i increase was blocked by torasemide (IC(50)=2.0+/-0.2x10(-8) and 2.7+/-0.6x10(-6) M, respectively). Furosemide inhibited the initial rise in [Ca(2+)]i induced by ET-1, with no effect on the second rise. The specific chloride (Cl(-)) channel blocker diphenylamine-2-carboxylate inhibits both ET-1-induced responses in VSMCs (IC(50)=8.0+/-0.3x10(-9) and 2.5+/-0.7x10(-7) M, respectively). CONCLUSIONS: These results suggest that the ability of loop diuretics to interfere with the vascular actions of ET-1 may involve different molecular mechanisms. The ability of torasemide to block the vasoconstrictive action of ET-1 could include an inhibitory action on Cl(-) channels.
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Appears in Collections:DA - CIMA - Servicios de apoyo - Biobanco - Artículos de revista
DA - CIMA - Cardiovasculares - Enfermedad vascular - Artículos de Revista
DA - CIMA - Cardiovasculares - Cardiopatía hipertensiva - Artículos de Revista

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