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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Neurociencias > Neuroanatomía ganglios basales > DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista >

Production of highly pure human glycosylated GDNF in a mammalian cell line
Authors: Ansorena, E. (Eduardo)
Garbayo, E. (Elisa)
Lanciego, J.L. (José Luis)
Aymerich, M.S. (María S.)
Blanco-Prieto, M.J. (María José)
Keywords: Therapeutic proteins
Purification of proteins
Glycosylated proteins
Neurotrophic factors
Parkinson’s disease
Issue Date: 2010
Publisher: Elsevier
Publisher version:
ISSN: 1873-3476
Citation: Ansorena E, Garbayo E, Lanciego JL, Aymerich MS, Blanco-Prieto MJ. Production of highly pure human glycosylated GDNF in a mammalian cell line. Int J Pharm 2010 Jan 29;385(1-2):6-11.
The administration of glial cell line-derived neurotrophic factor (GDNF) has emerged as a promising strategy for the treatment of several diseases of the nervous system as Parkinson's disease, amyotrophic lateral sclerosis, spinal cord injury and nerve regeneration as well as ocular diseases and drug addictions. A procedure for the purification of human recombinant glycosylated GDNF using a mammalian expression system as the source of the protein is discussed in the present paper. The neurotrophic factor was purified using cation exchange chromatography and gel filtration. A human cell line was chosen as the source of therapeutic protein, since a recombinant protein with a structure and glycosylation pattern equivalent to the native form is desirable for its prospective therapeutic utilization. The activity of the highly pure protein obtained was confirmed with a cell-based bioassay. The purified protein is suitable for its in vivo evaluation in animals and for possible subsequent clinical application.
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DA - CIMA - Neurociencias - Neuroanatomía ganglios basales - Artículos de Revista

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