Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Garciandia, A. (Ana) | - |
dc.creator | Lopez, R. (Rafael) | - |
dc.creator | Tisaire, J. (Javier) | - |
dc.creator | Arrazola, A. (Arantxa) | - |
dc.creator | Fortuño, A. (Ana) | - |
dc.creator | Bueno, J. (José) | - |
dc.creator | Diez-Martinez, J. (Javier) | - |
dc.date.accessioned | 2011-12-02T13:47:34Z | - |
dc.date.available | 2011-12-02T13:47:34Z | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Garciandia A, Lopez R, Tisaire J, Arrazola A, Fortuño A, Bueno J, et al. Enhanced Na(+)-H+ exchanger activity and NHE-1 mRNA expression in lymphocytes from patients with essential hypertension. Hypertension 1995 Mar;25(3):356-364. | es_ES |
dc.identifier.issn | 0194-911X | - |
dc.identifier.uri | https://hdl.handle.net/10171/20092 | - |
dc.description.abstract | It has been demonstrated that the activity of the sodium-proton exchanger (NHE-1 isoform) is increased in lymphocytes and other blood cells from patients with essential hypertension. In the present study, we investigated whether an increased level of NHE-1-specific mRNA in lymphocytes from patients with essential hypertension would explain the enhanced transport activity. Twenty-two hypertensive patients and 21 normotensive subjects were studied. Basal cytosolic pH was measured by the pH-sensitive fluorescent probe 2',7'-bis(2-carboxyethyl)-5(6)-carboxyfluorescein. Maximal sodium-proton exchange activity was determined by acidifying cell pH and measuring the initial rate of the net sodium-dependent proton efflux driven by an outward proton gradient. The transcript level of NHE-1 was measured by reverse transcription-polymerase chain reaction in comparison with a constitutively expressed reference gene (beta-actin). Intracellular pH was lower in hypertensive patients than normotensive subjects (7.34 +/- 0.01 versus 7.39 +/- 0.01, mean +/- SEM, P < .001). The maximal activity of the sodium-proton exchanger was higher in hypertensive patients than in normotensive subjects (1262 +/- 100 versus 881 +/- 56 mmol/L cells per hour, P < .01). NHE-1 mRNA was increased in hypertensive patients compared with normotensive subjects (ratio of NHE-1 mRNA to beta-actin mRNA, 0.16 +/- 0.01 versus 0.12 +/- 0.02, P < .05). These data suggest that the increased sodium-proton exchange activity in essential hypertension may be related to the de novo synthesis of exchanger protein. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American heart association | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Hypertension, essential | es_ES |
dc.subject | Lymphocytes | es_ES |
dc.subject | Na+-H+ exchanger | es_ES |
dc.subject | NHE-1 isoform | es_ES |
dc.title | Enhanced Na(+)-H+ exchanger activity and NHE-1 mRNA expression in lymphocytes from patients with essential hypertension | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1161/01.hyp.25.3.356 | es_ES |
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