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Development of the endocrine pancreas during larval phases of Rana temporaria. An immunocytochemical and ultrastructural study
Authors: Ortiz-de-Zarate, A. (A.)
Villaro, A.C. (Ana Cristina)
Etayo, J.C. (Juan Carlos)
Diaz-de-Rada, O. (O.)
Montuenga, L.M. (Luis M.)
Sesma, M.P. (María Pilar)
Vazquez, J.J. (Jesús Jaime)
Keywords: Pancreas, endocrine
Larval development
Serial thin/semithin sections
Rana temporaria (Anura)
Issue Date: 1991
Publisher: Springer Verlag
Publisher version:
ISSN: 1432-0878
Citation: Ortiz de Zarate A, Villaro AC, Etayo JC, Diaz de Rada O, Montuenga LM, Sesma P, et al. Development of the endocrine pancreas during larval phases of Rana temporaria. An immunocytochemical and ultrastructural study. Cell Tissue Res 1991 Apr;264(1):139-150.
The pancreatic endocrine component was studied at different stages of development in the tadpoles of Rana temporaria. The material was embedded in Epon, and serial semithin and thin sections were made in order to correlate ultrastructural features and tinctorial traits of the endocrine cells. Serial semithin sections were also stained with the peroxidase-antiperoxidase immunocytochemical method and with silver impregnations for argyrophilia and argentaffinity. In early larvae (legless tadpoles). A and B cells are present. Both can be found within ducts and exocrine tissue or, more frequently, in cellular clusters among the ducts and acini. These primitive islets are solid structures, surrounded but not penetrated by capillaries. Mitoses were observed in A and B cells. In the following phase (tadpoles with hindlegs), D and pancreatic polypeptide-immunoreactive cells are also present, as well as numerous endocrine cells scattered among exocrine tissue. There is also a change in the vascular-insular pattern: capillaries not only surround but also penetrate the endocrine group. The structure of the endocrine pancreas in older tadpoles is similar. Tinctorial traits and ultrastructural features of endocrine cells are described, and the origin of primitive islets is discussed.
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DA - CIMA - Oncología - Biomarcadores - Artículos de Revista

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