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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Biomarcadores > DA - CIMA - Oncología - Biomarcadores - Artículos de Revista >

In situ detection of AE2 anion-exchanger mRNA in the human liver
Autor(es) : Garcia, C. (Carmen)
Montuenga, L.M. (Luis M.)
Medina, J.F. (Juan Francisco)
Prieto, J. (Jesús)
Palabras clave : Anion exchange
Bicarbonate secretion
Bile-duct epithelial cells
Hepatocytes
Immunohistochemistry
Liver lymphocytes
T cells
Man
Fecha incorporación: 1998
Editorial : Springer Verlag
Versión del editor: http://www.springerlink.com/content/3e57vcr8jfc8f0yx/
ISSN: 1432-0878
Cita: Garcia C, Montuenga LM, Medina JF, Prieto J. In situ detection of AE2 anion-exchanger mRNA in the human liver. Cell Tissue Res 1998 Mar;291(3):481-488.
Resumen
Na+-independent anion exchangers, a family of membrane proteins that mediate electroneutral exchanges of chloride and bicarbonate ions across the cell membrane, are considered to be involved in intracellular pH regulation as well as in transepithelial acid/base transport. Previous immunohistochemical data have shown that anion-exchanger-2 (AE2) protein is expressed in the liver parenchyma, localizing at both the canaliculi and the luminal surfaces of intrahepatic bile ducts, where it may have a role in the biliary secretion of bicarbonate. In the present study, we have carried out in situ hybridization experiments on biopsies of human liver using three overlapping antisense anion-exchanger-2 riboprobes. Anion-exchanger-2 mRNA signals were localized mainly in the cytoplasm of terminal and interlobular bile-duct cells, whereas weaker signals were observed in bile-duct cells of larger intrahepatic ducts. Furthermore, some hepatocytes, mostly periportal, contained detectable anion-exchanger-2 mRNA signals in their cytoplasm. No hybridization signals were observed in controls with sense riboprobes, with omission of the antisense probe, or with treatment of the sections with RNase before hybridizations. Finally, intense anion-exchanger-2 hybridization signals were observed in lymphomononuclear cells in sinusoids and in portal infiltrates. Immunocytochemical data from reverse-phase sections suggest that these cells correspond to some of the CD45R+ (UCHL1+) T lymphocytes resident in the liver.
Enlace permanente: http://hdl.handle.net/10171/20151
Aparece en las colecciones: DA - CIMA - Oncología - Biomarcadores - Artículos de Revista
DA - Ciencias - HAP - Artículos de revista
DA - CUN - Medicina interna - Artículos de revista
DA - CUN - Hepatología - Artículos de revista
DA - Medicina - Medicina Interna - Artículos de revista

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Fichero:  54CGarcia et al_CellTissueRes98.pdf
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