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|Presence of leptin receptors in rat small intestine and leptin effect on sugar absorption|
|Authors: ||Lostao, M.P. (María Pilar)|
Urdaneta, E. (Elena)
Martinez-Anso, E. (Eduardo)
Barber, A. (Ana)
Martinez, J.A. (José Alfredo)
|Issue Date: ||1998|
|Publisher version: ||http://dx.doi.org/10.1016/S0014-5793(98)00110-0|
|Citation: ||Lostao MP, Urdaneta E, Martinez-Anso E, Barber A, Martinez JA. Presence of leptin receptors in rat small intestine and leptin effect on sugar absorption. FEBS Lett 1998 Feb 27;423(3):302-306.|
Leptin is involved in food intake and thermogenesis regulation. Since leptin receptor expression has been found in several tissues including small intestine, a possible role of leptin in sugar absorption by the intestine was investigated. Leptin inhibited D-galactose uptake by rat small intestinal rings 33% after 5 min of incubation. The inhibition increased to 56% after 30 min. However, neither at 5 min nor at 30 min did leptin prevent intracellular galactose accumulation. This leptin effect was accompanied by a decrease of the active sugar transport apparent Vmax (20 vs. 4.8 micromol/g wet weight 5 min) and apparent Km (15.8 vs. 5.3 mM) without any change in the phlorizin-resistant component. On the other hand, immunohistochemical experiments using anti-leptin monoclonal antibodies recognized leptin receptors in the plasma membrane of immune cells located in the lamina propria. These results indicate for the first time that leptin has a rapid inhibitory effect on sugar absorption and demonstrate the presence of leptin receptors in the intestinal mucosa.
|Permanent link: ||http://hdl.handle.net/10171/20252|
|Appears in Collections:||DA - CIMA - Terapia génica y Hepatología - Anticuerpos monoclonales - Artículos de Revista|
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