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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Oncología > Microambiente tumoral > DA - CIMA - Oncología - Microambiente tumoral - Artículos de revista >

Nitric oxide activates granule-associated DNase in human monocytes
Autor(es) : Pio, R. (Rubén)
Lopez-Zabalza, M.J. (María Jesús)
Rouzaut, A. (Ana)
Santiago, E. (Esteban)
Lopez-Moratalla, N. (Natalia)
Palabras clave : Nitric oxide
DNase activity
Human monocytes
Cytotoxic monocytes
Fecha incorporación: 1998
Editorial : Elsevier
Versión del editor: http://www.sciencedirect.com/science/article/pii/S1089860398901756
ISSN: 1089-8611
Cita: Pio R, Lopez-Zabalza MJ, Rouzaut A, Santiago E, Lopez-Moratalla N. Nitric oxide activates granule-associated DNase in human monocytes. Nitric Oxide 1998;2(3):165-173.
Resumen
Activated and differentiated human monocytes with a CD14+CD16+ phenotype were found to contain a DNase activity associated with secretion granules. Activated cells were obtained from patients with autoimmune diseases. Activation and differentiation of monocytes were also achieved after incubation of PBMC from healthy subjects with protein A (SpA) or immunopotentiating peptides. DNase activity corresponded to a 66-kDa protein, similar to that described in granules from T lymphocytes, active preferentially on double-strand DNA. DNA fragmentation activity increased when NO donors were present; the activity was higher in the presence of Ca2+, and at low pH values. The Ca2+-dependent activity was inhibited by Zn2+. NO-dependent activity was additive with that of Ca2+-dependent and it was not inhibited by Zn2+. Dithiothreitol did not modify the effect of NO on DNase activity. Incubation of PBMC in the presence of NMLA, an inhibitor of NO synthases, decreased this DNase activity. Data reported clearly suggest a regulatory role of NO in granule-associated DNase activity
Enlace permanente: http://hdl.handle.net/10171/20313
Aparece en las colecciones: DA - CIMA - Oncología - Biomarcadores - Artículos de Revista
DA - Ciencias - Bioquímica y Biología molecular - Artículos de Revista
DA - CIMA - Oncología - Microambiente tumoral - Artículos de revista

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Fichero:  NitricOxideBiolChem199823165.pdf
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