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Dendritic cells adhere to and transmigrate across lymphatic endothelium in response to IFN-α
Authors: Rouzaut, A. (Ana)
Garasa, S. (Saray)
Teijeira, A. (Álvaro)
Gonzalez, I. (Iranzu)
Martinez-Forero, I. (Iván)
Suarez, N. (Natalia)
Larrea, E. (Esther)
Alfaro, C. (Carlos)
Palazon, A. (Asís)
Dubrot, J. (Juan)
Hervas-Stubbs, S. (Sandra)
Melero, I. (Ignacio)
Keywords: Cell traffic
Lymphatic vessels
Issue Date: 2010
Publisher: Wiley Vch Verlag Berlin
Publisher version:
ISSN: 1521-4141
Citation: Rouzaut A, Garasa S, Teijeira A, Gonzalez I, Martinez-Forero I, Suarez N, et al. Dendritic cells adhere to and transmigrate across lymphatic endothelium in response to IFN-alpha. Eur J Immunol 2010 Nov;40(11):3054-3063.
Migration of DC into lymphatic vessels ferries antigenic cargo and pro-inflammatory stimuli into the draining LN. Given that tissues under the influence of viral infections produce type I IFN, it is conceivable that these cytokines enhance DC migration in order to facilitate an antiviral immune response. Cultured lymphatic endothelium monolayers pretreated with TNF-α were used to model this phenomenon under inflammatory conditions. DC differentiated in the presence of either IFN-α2b or IFN-α5 showed enhanced adhesion to cultured lymphatic endothelial cells. These pro-adhesive effects were mediated by DC, not the lymphatic endothelium, and correlated with increased DC transmigration across lymphatic endothelial cell monolayers. Transmigration was guided by chemokines acting on DC, and blocking experiments with mAb indicated a role for LFA-1. Furthermore, incubation of DC with IFN-α led to the appearance of active conformation epitopes on the CD11a integrin chains expressed by DC. Differentiation of mouse DC in the presence of IFN-α also increased DC migration from inflammed footpads toward popliteal LN. Collectively, these results indicate a role for type I IFN in directing DC toward LN under inflammatory conditions.
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Appears in Collections:DA - CIMA - Terapia génica y Hepatología - Inmunología clínica - Artículos de revista
DA - Medicina - Medicina Interna - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología terapia génica - Artículos de revista
DA - CUN - Área de Terapia Celular - Artículos de revista
DA - Ciencias - Bioquímica y Biología molecular - Artículos de Revista
DA - CIMA - Oncología - Microambiente tumoral - Artículos de revista

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