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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Anticuerpos monoclonales > DA - CIMA - Terapia génica y Hepatología - Anticuerpos monoclonales - Artículos de Revista >

The plasma membrane polarity of human biliary epithelial cells: in situ immunohistochemical analysis and functional implications
Autor(es) : Scoazec, J.Y. (Jean Yves)
Bringuier, A.F. (Annie-France)
Medina, J.F. (Juan Francisco)
Martinez-Anso, E. (Eduardo)
Veissiere, D. (Danielle)
Feldmann, G. (Gérard)
Housset, C. (Chantal)
Palabras clave : Human biliary epithelial cells
Immunohistochemistry
Membrane transport
Plasma membrane
Plasma membrane proteins
Polarity
Simple epithelial cells
Fecha incorporación: 1997
Editorial : Elsevier
Versión del editor: http://dx.doi.org/10.1016/S0168-8278(97)80419-9
ISSN: 0168-8278
Cita: Scoazec JY, Bringuier AF, Medina JF, Martinez-Anso E, Veissiere D, Feldmann G, et al. The plasma membrane polarity of human biliary epithelial cells: in situ immunohistochemical analysis and functional implications. J Hepatol 1997 Mar;26(3):543-553.
Resumen
BACKGROUND/AIMS: In transporting epithelia, like the biliary epithelium, most plasma membrane proteins present a polarized distribution, essential for the maintenance of the structural and functional properties of the epithelium. We therefore analyzed the expression of polarized plasma membrane proteins by human biliary epithelial cells in order to compare them with other transporting epithelial cells and to search for differences in plasma membrane protein expression between their different anatomical subsets. METHODS: We designed an in situ immunohistochemical study of the various anatomical compartments of the human biliary tract in order to assess the pattern of expression of selected polarized plasma membrane proteins, including integrin receptors, ectopeptidases, membrane transporters and GPI-linked proteins. RESULTS: All biliary epithelial cells expressed the same repertoire of integrins, except for integrin chain alpha5, restricted to the intra-hepatic compartments. All biliary epithelial cells expressed the following apical ectopeptidases: aminopeptidase-N, neutral-endopeptidase, dipeptidyl-peptidase IV. All biliary epithelial cells expressed the membrane transporter Na+ K+-ATPase, restricted to the basolateral domain, and the apical transporters CFTR and MDR-1. The apical AE2 anion exchanger was restricted to the small intra-hepatic bile ducts and the gallbladder. The GPI-linked protein protectin was basolateral in the intrahepatic bile ducts and apical in the gallbladder. CONCLUSIONS: The structural organization of the plasma membrane of biliary epithelial cells is very similar to that of other simple epithelia and exhibits a limited degree of heterogeneity.
Enlace permanente: http://hdl.handle.net/10171/20323
Aparece en las colecciones: DA - CIMA - Terapia génica y Hepatología - Anticuerpos monoclonales - Artículos de Revista

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