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dc.creatorMasyuk, A.I. (Anatoly I.)-
dc.creatorGradilone, S.A. (Sergio A.)-
dc.creatorBanales, J.M. (Jesús M.)-
dc.creatorHuang, B.Q. (Bing Q.)-
dc.creatorMasyuk, T.V. (Tatyana V.)-
dc.creatorLee, S.O. (Seung-Ok)-
dc.creatorSplinter, P.L. (Patrick L.)-
dc.creatorStroope, A. (Angela)-
dc.creatorLaRusso, N.F. (Nicholas F.)-
dc.identifier.citationMasyuk AI, Gradilone SA, Banales JM, Huang BQ, Masyuk TV, Lee SO, et al. Cholangiocyte primary cilia are chemosensory organelles that detect biliary nucleotides via P2Y12 purinergic receptors. Am J Physiol Gastrointest Liver Physiol 2008 Oct;295(4):G725-34.es_ES
dc.description.abstractCholangiocytes, the epithelial cells lining intrahepatic bile ducts, contain primary cilia, which are mechano- and osmosensory organelles detecting changes in bile flow and osmolality and transducing them into intracellular signals. Here, we asked whether cholangiocyte cilia are chemosensory organelles by testing the expression of P2Y purinergic receptors and components of the cAMP signaling cascade in cilia and their involvement in nucleotide-induced cAMP signaling in the cells. We found that P2Y(12) purinergic receptor, adenylyl cyclases (i.e., AC4, AC6, and AC8), and protein kinase A (i.e., PKA RI-beta and PKA RII-alpha regulatory subunits), exchange protein directly activated by cAMP (EPAC) isoform 2, and A-kinase anchoring proteins (i.e., AKAP150) are expressed in cholangiocyte cilia. ADP, an endogenous agonist of P2Y(12) receptors, perfused through the lumen of isolated rat intrahepatic bile ducts or applied to the ciliated apical surface of normal rat cholangiocytes (NRCs) in culture induced a 1.9- and 1.5-fold decrease of forskolin-induced cAMP levels, respectively. In NRCs, the forskolin-induced cAMP increase was also lowered by 1.3-fold in response to ATP-gammaS, a nonhydrolyzed analog of ATP but was not affected by UTP. The ADP-induced changes in cAMP levels in cholangiocytes were abolished by chloral hydrate (a reagent that removes cilia) and by P2Y(12) siRNAs, suggesting that cilia and ciliary P2Y(12) are involved in nucleotide-induced cAMP signaling. In conclusion, cholangiocyte cilia are chemosensory organelles that detect biliary nucleotides through ciliary P2Y(12) receptors and transduce corresponding signals into a cAMP response.es_ES
dc.publisherAmerican Physiological Societyes_ES
dc.subjectAdenylyl cyclaseses_ES
dc.subjectProtein kinase Aes_ES
dc.subjectExchange protein directly activated by cAMPes_ES
dc.subjectA-kinase anchoring protein 150es_ES
dc.titleCholangiocyte primary cilia are chemosensory organelles that detect biliary nucleotides via P2Y12 purinergic receptorses_ES

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