Full metadata record
DC Field | Value | Language |
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dc.creator | Diaz-Valdes, N. (Nancy) | - |
dc.creator | Manterola, L. (Lorea) | - |
dc.creator | Belsue, V. (Virginia) | - |
dc.creator | Riezu-Boj, J.I. (José Ignacio) | - |
dc.creator | Larrea, E. (Esther) | - |
dc.creator | Echeverria, I. (Itziar) | - |
dc.creator | Llopiz, D. (Diana) | - |
dc.creator | Lopez-Sagaseta, J. (Jacinto) | - |
dc.creator | Lerat, H. (Hervé) | - |
dc.creator | Pawlotsky, J.M. (Jean-Michel) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.creator | Lasarte, J.J. (Juan José) | - |
dc.creator | Borras-Cuesta, F. (Francisco) | - |
dc.creator | Sarobe, P. (Pablo) | - |
dc.date.accessioned | 2011-12-22T13:43:20Z | - |
dc.date.available | 2011-12-22T13:43:20Z | - |
dc.date.issued | 2011 | - |
dc.identifier.citation | Diaz-Valdes N, Manterola L, Belsue V, Riezu-Boj JI, Larrea E, Echeverria I, et al. Improved dendritic cell-based immunization against hepatitis C virus using peptide inhibitors of interleukin 10. Hepatology 2011 Jan;53(1):23-31. | es_ES |
dc.identifier.issn | 0270-9139 | - |
dc.identifier.uri | https://hdl.handle.net/10171/20359 | - |
dc.description.abstract | The high levels of interleukin 10 (IL-10) present in chronic hepatitis C virus (HCV) infection have been suggested as responsible for the poor antiviral cellular immune responses found in these patients. To overcome the immunosuppressive effect of IL-10 on antigen-presenting cells such as dendritic cells (DCs), we developed peptide inhibitors of IL-10 to restore DC functions and concomitantly induce efficient antiviral immune responses. Two IL-10-binding peptides (p9 and p13) were selected using a phage-displayed library and their capacity to inhibit IL-10 was assessed in a bioassay and in STAT-3 (signal transducer and activator of transcription 3) phosphorylation experiments in vitro. In cultures of human leukocytes where HCV core protein induces the production of IL-10, p13 restored the ability of plasmacytoid DC to produce interferon alpha (IFN-α) after Toll-like receptor 9 (TLR9) stimulation. Similarly, when myeloid DCs were stimulated with CD40L in the presence of HCV core, p9 enhanced IL-12 production by inhibiting HCV core-induced as well as CD40L-induced IL-10. Moreover, in vitro, p13 potentiated the effect of maturation stimuli on human and murine DC, increasing their IL-12 production and stimulatory activity, which resulted in enhanced proliferation and IFN-γ production by responding T-cells. Finally, immunization with p13-treated murine DC induced stronger anti-HCV T-cell responses not only in wildtype mice but also in HCV transgenic mice and in mice transiently expressing HCV core in the liver. CONCLUSION: These results suggest that IL-10 inhibiting peptides may have important applications to enhance anti-HCV immune responses by restoring the immunostimulatory capabilities of DC. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley blackwell | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Dendritic Cells/immunology | es_ES |
dc.subject | Hepacivirus/immunology | es_ES |
dc.subject | Interleukin-10/antagonists & inhibitors | es_ES |
dc.subject | Interleukin-12/biosynthesis | es_ES |
dc.title | Improved dendritic cell-based immunization against hepatitis C virus using peptide inhibitors of interleukin 10 | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | 10.1002/hep.23980 | es_ES |
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