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Campo DC Valor Lengua/Idioma
dc.creatorOchoa, L. (Laura)-
dc.creatorOtano, I. (Itziar)-
dc.creatorVales, A. (África)-
dc.creatorOlagüe, C. (Cristina)-
dc.creatorSarobe, P. (Pablo)-
dc.creatorLasarte, J.J. (Juan José)-
dc.creatorMenne, S. (Stephan)-
dc.creatorPrieto, J. (Jesús)-
dc.creatorGonzalez-Aseguinolaza, G. (Gloria)-
dc.identifier.citationOchoa-Callejero L, Otano I, Vales A, Olagüe C, Sarobe P, Lasarte JJ, et al. Identification of CD4+ and CD8+ T cell epitopes of woodchuck hepatitis virus core and surface antigens in BALB/c mice. Vaccine 2010 Jul 19;28(32):5323-5331.es_ES
dc.description.abstractA therapeutic vaccine against chronic hepatitis B virus (HBV) infection requires the development of a strong and multispecific Th1 cell immune response. Woodchucks chronically infected with the woodchuck hepatitis virus (WHV) closely resemble HBV infection and represent the best animal model for this hepadnavirus-induced disease. Using the BIMAS "HLA Peptide Binding Predictions" program, we have identified and further characterized novel H-2(d)-restricted CD8+ epitopes within the WHV core (peptides C#12-21, C#18-32, C#19-27, C#61-69) and surface antigens (peptides preS2#10-18, preS2#27-35, S#76-84, S#133-140 and S#257-265), respectively. These peptides bind to H-2(d) with high efficiency and upon immunization of mice with peptide and Freund's adjuvant they induce the development of IFN-gamma producing T cells. More importantly, WHV core peptides C#19-27 and C#61-69 and WHV surface peptides S#133-140 and S#257-265 were also recognized by CD8+ T cells after immunization of mice with DNA/PEI nanoparticles. Direct stimulation of splenocytes obtained from such DNA-immunized mice with peptides C#18-32, S#76-84, and S#257-265 resulted in significant production of IFN-gamma. Thus, we have identified T cell determinants in mice from WHV core and surface antigens that have important value for designing and evaluating an effective vaccine against hepadnavirus infection.es_ES
dc.subjectWoodchuck hepatitis viruses_ES
dc.subjectT cell epitopeses_ES
dc.subjectHepatitis B viruses_ES
dc.subjectChronic hepatitis infectiones_ES
dc.subjectDNA vaccinees_ES
dc.titleIdentification of CD4+ and CD8+ T cell epitopes of woodchuck hepatitis virus core and surface antigens in BALB/c micees_ES

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