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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.creator | Lasarte, J.J. (Juan José) | - |
dc.creator | Casares, N. (Noelia) | - |
dc.creator | Gorraiz, M. (Marta) | - |
dc.creator | Hervas-Stubbs, S. (Sandra) | - |
dc.creator | Arribillaga, L. (Laura) | - |
dc.creator | Mansilla, C. (Cristina) | - |
dc.creator | Durantez, M. (Maika) | - |
dc.creator | Llopiz, D. (Diana) | - |
dc.creator | Sarobe, P. (Pablo) | - |
dc.creator | Borras-Cuesta, F. (Francisco) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.creator | Leclerc, C. (Claude) | - |
dc.date.accessioned | 2012-01-16T08:29:33Z | - |
dc.date.available | 2012-01-16T08:29:33Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Lasarte JJ, Casares N, Gorraiz M, Hervas-Stubbs S, Arribillaga L, Mansilla C, et al. The extra domain A from fibronectin targets antigens to TLR4-expressing cells and induces cytotoxic T cell responses in vivo. J Immunol 2007 Jan 15;178(2):748-756 | es_ES |
dc.identifier.issn | 0022-1767 | - |
dc.identifier.uri | https://hdl.handle.net/10171/20425 | - |
dc.description.abstract | Vaccination strategies based on the in vivo targeting of Ags to dendritic cells (DCs) are needed to improve the induction of specific T cell immunity against tumors and infectious agents. In this study, we have used a recombinant protein encompassing the extra domain A from fibronectin (EDA), an endogenous ligand for TLR4, to deliver Ags to TLR4-expressing DC. The purified EDA protein was shown to bind to TLR4-expressing HEK293 cells and to activate the TLR4 signaling pathway. EDA also stimulated the production by DC of proinflammatory cytokines such as IL-12 or TNF-alpha and induced their maturation in vitro and in vivo. A fusion protein between EDA and a cytotoxic T cell epitope from OVA efficiently presented this epitope to specific T cells and induced the in vivo activation of a strong and specific CTL response. Moreover, a fusion protein containing EDA and the full OVA also improved OVA presentation by DC and induced CTL responses in vivo. These EDA recombinant proteins protected mice from a challenge with tumor cells expressing OVA. These results strongly suggest that the fibronectin extra domain A may serve as a suitable Ag carrier for the development of antiviral or antitumoral vaccines. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American association of immunologists | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Fibronectins/metabolism | es_ES |
dc.subject | T-Lymphocytes, Cytotoxic/metabolism | es_ES |
dc.subject | Toll-Like Receptor 4/metabolism | es_ES |
dc.title | The extra domain A from fibronectin targets antigens to TLR4-expressing cells and induces cytotoxic T cell responses in vivo | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.jimmunol.org/content/178/2/748.long | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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