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|Identification and characterization of a T-helper peptide from carcinoembryonic antigen|
|Authors: ||Ruiz, M. (Marta)|
Kobayashi, H. (Hiroya)
Lasarte, J.J. (Juan José)
Prieto, J. (Jesús)
Borras-Cuesta, F. (Francisco)
Celis, E. (Esteban)
Sarobe, P. (Pablo)
|Keywords: ||Carcinoembryonic Antigen/chemistry|
|Issue Date: ||2004|
|Publisher: ||American association for cancer research|
|Publisher version: ||http://dx.doi.org/10.1158/1078-0432.CCR-03-0476|
|Citation: ||Ruiz M, Kobayashi H, Lasarte JJ, Prieto J, Borras-Cuesta F, Celis E, at al. Identification and characterization of a T-helper peptide from carcinoembryonic antigen. Clin Cancer Res 2004 Apr 15;10(8):2860-2867.|
PURPOSE: The purpose of this research was to identify promiscuous T-helper cell determinants (THd) from carcinoembryonic antigen (CEA) to be used to prime T-cell help for cancer therapy. CEA was selected because this antigen is expressed in an important variety of carcinomas.
EXPERIMENTAL DESIGN: Potential promiscuous THd from CEA were predicted using available computer algorithms. Predicted peptides were synthesized and tested in binding experiments to different HLA-DR molecules. Binder peptides were then used to prime T-cell responses both in vitro and in vivo.
RESULTS: Twenty 15-mer peptides from CEA were predicted to bind to different HLA-DR molecules. The promiscuous character of these peptides was demonstrated in binding experiments. Fifteen of 20 peptides tested were able to bind to HLA-DR4, but only CEA (625-639) was shown to be presented after processing of recombinant CEA. CEA (625-639) was also found to be presented by HLA-DR53. Moreover, immunization of HLA-DR4 transgenic mice with CEA (625-639) in conjunction with class I epitope OVA (257-264), induced a CTL response specific of OVA (257-264).
CONCLUSIONS: CEA (625-639) might be a relevant promiscuous THd peptide for cancer therapy.
|Permanent link: ||http://hdl.handle.net/10171/20434|
|Appears in Collections:||DA - Medicina - Medicina Interna - Artículos de revista|
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista
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