Identification of HLA-B27-restricted cytotoxic T lymphocyte epitope from carcinoembryonic antigen
Keywords: 
Cytotoxic T lymphocyte
Carcinoembryonic antigen
HLA-B27
Peptides
Tumor
Issue Date: 
2002
Publisher: 
Wiley-Blackwell
ISBN: 
0020-7136
Citation: 
Huarte E, Sarobe P, Lasarte JJ, Brem G, Weiss EH, Prieto J, et al. Identification of HLA-B27-restricted cytotoxic T lymphocyte epitope from carcinoembryonic antigen. Int J Cancer 2002 Jan 1;97(1):58-63.
Abstract
Characterization of epitopes recognized by cytotoxic T lymphocytes (CTLs) in the sequence of tumor antigens is an important step in the development of tumor therapies. Because carcinoembryonic antigen (CEA) is a protein expressed in a high number of epithelial tumors, it is an interesting target to study. We screened for the presence of HLA-B27-restricted CTL epitopes from CEA by studying the binding to HLA-B27 of 31 synthetic peptides predicted to bind to this molecule. This afforded 16 peptides with moderate or high binding affinity. Immunization of HLA-B27 transgenic mice with the best binder peptides yielded 4 immunogenic peptides: CEA(9-17), CEA(9-18), CEA(138-146) and CEA(360-369). However, splenocytes from mice immunized with a vaccinia virus-expressing CEA recognized only CEA(9-18). These CTLs were of the CD8(+) phenotype, which upon stimulation with peptide specifically produced IFN-gamma. Moreover, they did not cross-react against peptides of region 9-18 from proteins of the CEA family. Our results show that CEA(9-18) may induce specific CTL responses against CEA.

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