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Levels of phytosterol oxides in enriched and nonenriched spreads: application of a thin-layer chromatography-gas chromatography methodology
Authors: Cochillo, A. (Ana)
Cercaci, L. (Luisito)
Ansorena, D. (Diana)
Rodriguez-Estrada, M.T. (María Teresa)
Lercker, G. (Giovanni)
Astiasarán, I. (Iciar)
Keywords: Phytosterol-enriched spread
Phytosterols oxidation products
Functional foods
Issue Date: 2005
Publisher: American Chemical Society
Publisher version:
ISSN: 0021-8561
Citation: Conchillo A, Cercaci L, Ansorena D, Rodriguez-Estrada MT, Lercker G, Astiasaran I. Levels of phytosterol oxides in enriched and nonenriched spreads: Application of a thin-layer chromatography-gas chromatography methodology. J Agric Food Chem. 2005 Oct 5;53(20):7844-50.
The content of phytosterol oxidation products (POPs) in enriched and nonenriched commercial spreads was evaluated by thin-layer chromatography-gas chromatography (TLC-GC). Oxides of beta-sitosterol, campesterol, and stigmasterol were produced by thermo-oxidation (7-hydroxy, 7-keto, and epoxy derivatives) and chemical synthesis (triol derivatives), which were then separated and identified by TLC-GC. Their identification was further confirmed by GC-mass spectrometry (GC-MS). The total amounts of phytosterols found were 6.07 and 0.33 g/100 g of sample in phytosterol-enriched and nonenriched spread, respectively, whereas the total POPs contents were 45.60 and 13.31 mg/kg of sample in the enriched and nonenriched products. The main POPs found were the 7-keto derivatives of all phytosterols analyzed; 7-ketositosterol was the most abundant one (14.96 and 5.93 mg/kg of sample in phytosterol-enriched and nonenriched spread). No beta-epoxy and triol derivatives were detected in both types of samples. The enriched spread presented a lower phytosterol oxidation rate (0.07%) than the nonenriched one (0.41%).
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