Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Lopez-Sanchez, L.M. (Laura M.) | - |
dc.creator | Corrales, F.J. (Fernando José) | - |
dc.creator | Mata, M. (Manuel) de la | - |
dc.creator | Muntane, J. (Jordi) | - |
dc.creator | Rodriguez-Ariza, A. (Antonio) | - |
dc.date.accessioned | 2012-04-02T07:51:11Z | - |
dc.date.available | 2012-04-02T07:51:11Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Lopez-Sanchez LM, Corrales FJ, De La Mata M, Muntane J, Rodriguez-Ariza A. Detection and proteomic identification of S-nitrosated proteins in human hepatocytes. Methods Enzymol 2008;440:273-281. | es_ES |
dc.identifier.issn | 1557-7988 | - |
dc.identifier.uri | https://hdl.handle.net/10171/21484 | - |
dc.description.abstract | The S-nitrosation of protein thiols is a redox-based posttranslational modification that modulates protein function and cell phenotype. Although the detection of S-nitrosated proteins is problematical because of the lability of S-nitrosothiols, an increasing range of proteins has been shown to undergo S-nitrosation with the improvement of molecular tools. This chapter describes the methodology used to identify potential targets of S-nitrosation in cultured primary human hepatocytes using proteomic approaches. This methodology is based on the biotin switch method, which labels S-nitrosated proteins with an affinity tag, allowing their selective detection and proteomic identification. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Hepatocytes/metabolism | es_ES |
dc.subject | Proteins/analysis | es_ES |
dc.subject | Proteins/metabolism | es_ES |
dc.subject | Proteomics/methods | es_ES |
dc.subject | S-Nitrosothiols/analysis | es_ES |
dc.subject | S-Nitrosothiols/metabolism | es_ES |
dc.title | Detection and proteomic identification of S-nitrosated proteins in human hepatocytes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0076687907008178 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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