Registro completo de metadatos
Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.creator | Perez-Mato, I. (Isabel) | - |
dc.creator | Castro, C. (Carmen) | - |
dc.creator | Ruiz, F.A. (Félix A.) | - |
dc.creator | Corrales, F.J. (Fernando José) | - |
dc.creator | Mato, J.M. (José María) | - |
dc.date.accessioned | 2012-04-03T11:26:02Z | - |
dc.date.available | 2012-04-03T11:26:02Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Perez-Mato I, Castro C, Ruiz FA, Corrales FJ, Mato JM. Methionine adenosyltransferase S-nitrosylation is regulated by the basic and acidic amino acids surrounding the target thiol. J Biol Chem 1999 Jun 11;274(24):17075-17079. | es_ES |
dc.identifier.issn | 1083-351X | - |
dc.identifier.uri | https://hdl.handle.net/10171/21546 | - |
dc.description.abstract | S-Adenosylmethionine serves as the methyl donor for many biological methylation reactions and provides the propylamine group for the synthesis of polyamines. S-Adenosylmethionine is synthesized from methionine and ATP by the enzyme methionine adenosyltransferase. The cellular factors regulating S-adenosylmethionine synthesis have not been well defined. Here we show that in rat hepatocytes S-nitrosoglutathione monoethyl ester, a cell-permeable nitric oxide donor, markedly reduces cellular S-adenosylmethionine content via inactivation of methionine adenosyltransferase by S-nitrosylation. Removal of the nitric oxide donor from the incubation medium leads to the denitrosylation and reactivation of methionine adenosyltransferase and to the rapid recovery of cellular S-adenosylmethionine levels. Nitric oxide inactivates methionine adenosyltransferase via S-nitrosylation of cysteine 121. Replacement of the acidic (aspartate 355) or basic (arginine 357 and arginine 363) amino acids located in the vicinity of cysteine 121 by serine leads to a marked reduction in the ability of nitric oxide to S-nitrosylate and inactivate hepatic methionine adenosyltransferase. These results indicate that protein S-nitrosylation is regulated by the basic and acidic amino acids surrounding the target cysteine. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Society for Biochemistry and Molecular Biology | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Glutathione/analogs & derivatives | es_ES |
dc.subject | Liver/enzymology | es_ES |
dc.subject | Methionine Adenosyltransferase/metabolism | es_ES |
dc.subject | Nitroso Compounds/metabolism | es_ES |
dc.title | Methionine adenosyltransferase S-nitrosylation is regulated by the basic and acidic amino acids surrounding the target thiol | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.jbc.org/content/274/24/17075 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
Ficheros en este ítem:
Estadísticas e impacto
Los ítems de Dadun están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.