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dc.creatorBorras-Cuesta, F. (Francisco)-
dc.creatorGolvano, J.J. (José Javier)-
dc.creatorSarobe, P. (Pablo)-
dc.creatorLasarte, J.J. (Juan José)-
dc.creatorPrieto, I. (Inés)-
dc.creatorSzabo, A. (A)-
dc.creatorGuillaume, J.L. (J. L.)-
dc.creatorGuillet, J.G. (J. G.)-
dc.date.accessioned2012-04-04T07:19:33Z-
dc.date.available2012-04-04T07:19:33Z-
dc.date.issued1991-
dc.identifier.citationBorras-Cuesta F, Golvano J, Sarobe P, Lasarte JJ, Prieto I, Szabo A, et al. Insights on the amino acid side-chain interactions of a synthetic T-cell determinant. Biologicals 1991 Jul;19(3):187-190.es_ES
dc.identifier.issn1095-8320-
dc.identifier.urihttp://hdl.handle.net/10171/21582-
dc.description.abstractThe effect of single amino acid substitutions at positions 18 and 20 on the T-cell determinant (TD) character of peptide p12-26 from lambda repressor protein and on its recognition by a monoclonal antibody was studied by means of 40 synthetic peptides of a length of 15 amino acids. ELISA competition experiments showed that the identity of amino acid at position 20 is very important for antibody recognition, whereas that of amino acid at position 18 is much less important. In contrast, both Leu 18 and Ala 20 are important residues in defining the TD character of peptide p12-26. The most tolerated replacements, ordered in increasing disrupting power are: Ala 20 by Cys, Ser or Gly and Leu 18 by Ile or Val. Any other amino acid replacement completely abolishes the TD capacity of peptide p12-26. The peptides used in this study were synthesized using a multiple solid-phase peptide synthesizer newly designed. Their purity was very high as shown by amino acid sequence experiments.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectDNA-Binding Proteinses_ES
dc.subjectEpitopes/chemistryes_ES
dc.subjectPeptides/immunologyes_ES
dc.subjectT-Lymphocytes/immunologyes_ES
dc.titleInsights on the amino acid side-chain interactions of a synthetic T-cell determinantes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/104510569190033Ges_ES
dc.type.driverinfo:eu-repo/semantics/articlees_ES

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