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|Identification of an antigenic epitope for helper T lymphocytes from carcinoembryonic antigen|
|Authors: ||Kobayashi, H. (Hiroya)|
Omiya, R. (Ryusuke)
Ruiz, M. (Marta)
Huarte, E. (Eduardo)
Sarobe, P. (Pablo)
Lasarte, J.J. (Juan José)
Herraiz-Bayod, M.J. (Maite J.)
Sangro, B. (Bruno)
Prieto, J. (Jesús)
Borras-Cuesta, F. (Francisco)
Celis, E. (Esteban)
|Keywords: ||Carcinoembryonic Antigen/immunology|
|Issue Date: ||2002|
|Publisher: ||American Association for Cancer Research|
|Publisher version: ||http://clincancerres.aacrjournals.org/content/8/10/3219|
|Citation: ||Kobayashi H, Omiya R, Ruiz M, Huarte E, Sarobe P, Lasarte JJ, et al. Identification of an antigenic epitope for helper T lymphocytes from carcinoembryonic antigen. Clin Cancer Res 2002 Oct;8(10):3219-3225.|
The product of the carcinoembryonic antigen (CEA) gene is an attractive candidate for T-cell-based immunotherapy because it is frequently expressed in epithelial solid carcinomas. Although many CEA peptide epitopes capable of stimulating CTLs have been identified, no MHC class II-restricted T helper epitope has yet been reported. Experimental Design: The amino acid sequence of CEA was examined for the presence of potential T helper epitopes, and candidate peptides were used to stimulate in vitro T-cell responses.
We describe here that using an algorithm to identify promiscuous helper T-cell epitopes, a peptide of CEA occupying residue positions 653 to 667 (CEA(653-667)), was effective in inducing in vitro T helper responses in the context of the HLA-DR4, HLA-DR7, and HLA-DR 9 alleles. Most significantly, some of the peptide-reactive helper T lymphocytes were also capable of recognizing naturally processed antigen in the form of recombinant CEA protein or cell lysates from tumors that express CEA. Interestingly, the newly identified helper T-cell epitope was found to overlap with a previously described HLA-A24-restricted CTL epitope, CEA(652-660), which could facilitate the development of a therapeutic vaccine capable of eliciting both CTL and T helper responses in patients suffering from epithelial carcinomas.
These results indicate that T helper lymphocytes are capable of recognizing CEA as a tumor antigen and that epitope CEA(653-667) could be used for immunotherapy against tumors expressing CEA.
|Permanent link: ||http://hdl.handle.net/10171/21600|
|Appears in Collections:||DA - Medicina - Medicina Interna - Artículos de revista|
DA - CIMA - Terapia génica y Hepatología - Inmunología hepatitis virales - Artículos de revista
DA - CIMA - Terapia génica y Hepatología - Inmunología experimental - Artículos de revista
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