Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Prieto, I. (Isidro) | - |
dc.creator | Hervas-Stubbs, S. (Sandra) | - |
dc.creator | Garcia-Granero, M. (Marta) | - |
dc.creator | Berasain, C. (Carmen) | - |
dc.creator | Riezu-Boj, J.I. (José Ignacio) | - |
dc.creator | Lasarte, J.J. (Juan José) | - |
dc.creator | Sarobe, P. (Pablo) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.creator | Borras-Cuesta, F. (Francisco) | - |
dc.date.accessioned | 2012-04-17T14:18:51Z | - |
dc.date.available | 2012-04-17T14:18:51Z | - |
dc.date.issued | 1995 | - |
dc.identifier.citation | Prieto I, Hervas-Stubbs S, Garcia-Granero M, Berasain C, Riezu-Boj JI, Lasarte JJ, et al. Simple strategy to induce antibodies of distinct specificity: application to the mapping of gp120 and inhibition of HIV-1 infectivity. Eur J Immunol 1995 Apr;25(4):877-883. | es_ES |
dc.identifier.issn | 1521-4141 | - |
dc.identifier.uri | https://hdl.handle.net/10171/21667 | - |
dc.description.abstract | In this study 96 15-mer peptides encompassing the entire sequence of HIV-1 gp120 were synthesized and used to immunize BALB/c mice (i) alone or (ii) in conjunction with the T helper cell determinant FISEAIIHVLHSR (FIS) from sperm whale myoglobin, which is well recognized by major histocompatibility complex (MHC) class II molecules of BALB/c. Of these peptides 39 were immunogenic per se and 57 were not. Out of the 57 non-immunogenic peptides 53 could be rendered immunogenic with the second immunization protocol. With the exception of 4 cases, the anti-peptide antibody titers induced in (ii) were equal (14 cases) or higher (78 cases) than those induced in (i). From the 96 anti-peptide antibodies tested, 12 were able to recognize recombinant gp120 with good antibody titers, a result in agreement with previously identified B cell epitopes from gp120 by anti-peptide antibodies induced with longer peptides conjugated to a carrier protein. Moreover, 4 of the 12 anti-peptide antisera that recognized gp 120 were able to neutralize HIV-1 infectivity in vitro, showing that the strategy of co-immunization with FIS may afford functional antibodies. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Wiley-VCH Verlag Berlin | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | T helper cell determinant | es_ES |
dc.subject | B cell determinant | es_ES |
dc.subject | HIV-1 | es_ES |
dc.subject | gp120 | es_ES |
dc.subject | Neutralizing antibodies | es_ES |
dc.title | Simple strategy to induce antibodies of distinct specificity: application to the mapping of gp120 and inhibition of HIV-1 infectivity | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://bit.ly/Ifl9N7 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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