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dc.creatorSarobe, P. (Pablo)-
dc.creatorLasarte, J.J. (Juan José)-
dc.creatorGolvano, J.J. (José Javier)-
dc.creatorPrieto, I. (Isidro)-
dc.creatorGullon, A. (Arturo)-
dc.creatorSoto, M.J. (María José)-
dc.creatorLabarga, P. (Pablo)-
dc.creatorPrieto, J. (Jesús)-
dc.creatorBorras-Cuesta, F. (Francisco)-
dc.date.accessioned2012-04-17T14:41:41Z-
dc.date.available2012-04-17T14:41:41Z-
dc.date.issued1994-
dc.identifier.citationSarobe P, Lasarte JJ, Golvano JJ, Prieto I, Gullon A, Soto MJ, et al. Induction of neutralizing antibodies against human immunodeficiency virus type 1 using synthetic peptide constructs containing an immunodominant T-helper cell determinant from vpr. J Acquir Immune Defic Syndr 1994 Jul;7(7):635-640.es_ES
dc.identifier.issn0894-9255-
dc.identifier.urihttp://hdl.handle.net/10171/21678-
dc.description.abstractIdentification of immunodominant T-helper-cell determinants after natural infection is an important step in the design of immunogens for potential use in vaccination. Using cells from human immunodeficiency virus type 1 (HIV-1)-infected individuals and a panel of peptides encompassing the sequence of the regulatory protein vpr from HIV-1, we identified the T-helper determinant QLLFIHFRIGCRHSR, which is active in 37.5% of these individuals. To gain insight on the efficacy of this peptide in helping induce neutralizing antibodies against a B-cell determinant (BD), we synthesized constructs containing B- and T-cell determinants and tested them in BALB/c mice, the highest responders to the T-cell determinant moiety among several strains tested. These immunogens induced antibodies against two chosen B-cell determinants from HIV-1IIIB gp160 (amino acids 310-322 from the V3 loop of gp120 and 736-751 from gp41) that were able to neutralize HIV-1 infection in vitro. The highest neutralization titer against HIV-1IIIB was obtained by immunization with the homopolymer of the construct containing the T-cell epitope from vpr and the B-cell epitope from the V3 loop. We believe that the immunodominant T-cell determinant from vpr is a promising epitope to consider in the design of future peptide vaccines.es_ES
dc.language.isoenges_ES
dc.publisherRaven Presses_ES
dc.rightsinfo:eu-repo/semantics/closedAccess-
dc.subjectGene Products, vpr/immunologyes_ES
dc.subjectHIV Antibodies/biosynthesises_ES
dc.subjectHIV-1/immunologyes_ES
dc.subjectImmunodominant Epitopes/immunologyes_ES
dc.subjectT-Lymphocytes, Helper-Inducer/immunologyes_ES
dc.titleInduction of neutralizing antibodies against human immunodeficiency virus type 1 using synthetic peptide constructs containing an immunodominant T-helper cell determinant from vpres_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://journals.lww.com/jaids/Abstract/1994/07000/Induction_of_Neutralizing_Antibodies_Against_Human.1.aspxes_ES
dc.type.driverinfo:eu-repo/semantics/articlees_ES

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