Full metadata record
DC FieldValueLanguage
dc.creatorZabala, M. (Maider)-
dc.creatorLasarte, J.J. (Juan José)-
dc.creatorPerret, C. (Christine)-
dc.creatorSola, J. (Josu)-
dc.creatorBerraondo, P. (Pedro)-
dc.creatorAlfaro, M. (Maite)-
dc.creatorLarrea, E. (Esther)-
dc.creatorPrieto, J. (Jesús)-
dc.creatorKramer, M.G. (María Gabriela)-
dc.date.accessioned2012-04-23T07:41:09Z-
dc.date.available2012-04-23T07:41:09Z-
dc.date.issued2007-
dc.identifier.citationZabala M, Lasarte JJ, Perret C, Sola J, Berraondo P, Alfaro M, et al. Induction of immunosuppressive molecules and regulatory T cells counteracts the antitumor effect of interleukin-12-based gene therapy in a transgenic mouse model of liver cancer. J Hepatol 2007 Dec;47(6):807-815.es_ES
dc.identifier.issn1600-0641-
dc.identifier.urihttp://hdl.handle.net/10171/21720-
dc.description.abstractBACKGROUND/AIMS: Hepatocellular carcinoma (HCC) often lacks curative treatment; therefore new efficient therapies are needed. In this work we aimed at evaluating the antitumor effect of interleukin-12 (IL-12)-based gene therapy on HCC occurring spontaneously in mice. METHODS: A plasmid-vector expressing IL-12 in a liver-specific and doxycycline (Dox)-inducible manner was transferred by hydrodynamic injection to the liver of L-PK/c-myc mice with HCC. IL-12 expression was induced by administering Dox (3 cycles of 1 month duration separated by 1 month rest). RESULTS: Dox administration increased serum IL-12 and IFN-gamma and induced tumor lymphocytic infiltration in all treated mice which was accompanied by tumor stabilization or regression in 40% of animals. The antitumor effect did not correlate with levels of IL-12 or IFN-gamma nor with the intensity of tumor mononuclear infiltration. However, tumors from non-responder mice showed more abundance of Foxp3+ regulatory T cells and higher expression of the immunosuppressive molecules PD-1, PD-L1, VEGF, CTLA-4, IDO, and IL-10 than those that responded to therapy. CONCLUSIONS: Although long-term induction of IL-12 expression in the liver can inhibit HCC growth, the efficacy of the treatment appears to be limited by the activation of immunosuppressive mechanisms.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/closedAccess-
dc.subjectGene therapyes_ES
dc.subjectInterleukin-12es_ES
dc.subjectRegulatory T cellses_ES
dc.subjectFoxp3es_ES
dc.subjectHepatocellular carcinomaes_ES
dc.subjectTet-on systemes_ES
dc.titleInduction of immunosuppressive molecules and regulatory T cells counteracts the antitumor effect of interleukin-12-based gene therapy in a transgenic mouse model of liver canceres_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0168827807005065es_ES
dc.type.driverinfo:eu-repo/semantics/articlees_ES

Files in This Item:
There are no files associated with this item.


Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.