Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Zabala, M. (Maider) | - |
dc.creator | Lasarte, J.J. (Juan José) | - |
dc.creator | Perret, C. (Christine) | - |
dc.creator | Sola, J. (Josu) | - |
dc.creator | Berraondo, P. (Pedro) | - |
dc.creator | Alfaro, M. (Maite) | - |
dc.creator | Larrea, E. (Esther) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.creator | Kramer, M.G. (María Gabriela) | - |
dc.date.accessioned | 2012-04-23T07:41:09Z | - |
dc.date.available | 2012-04-23T07:41:09Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Zabala M, Lasarte JJ, Perret C, Sola J, Berraondo P, Alfaro M, et al. Induction of immunosuppressive molecules and regulatory T cells counteracts the antitumor effect of interleukin-12-based gene therapy in a transgenic mouse model of liver cancer. J Hepatol 2007 Dec;47(6):807-815. | es_ES |
dc.identifier.issn | 1600-0641 | - |
dc.identifier.uri | https://hdl.handle.net/10171/21720 | - |
dc.description.abstract | BACKGROUND/AIMS: Hepatocellular carcinoma (HCC) often lacks curative treatment; therefore new efficient therapies are needed. In this work we aimed at evaluating the antitumor effect of interleukin-12 (IL-12)-based gene therapy on HCC occurring spontaneously in mice. METHODS: A plasmid-vector expressing IL-12 in a liver-specific and doxycycline (Dox)-inducible manner was transferred by hydrodynamic injection to the liver of L-PK/c-myc mice with HCC. IL-12 expression was induced by administering Dox (3 cycles of 1 month duration separated by 1 month rest). RESULTS: Dox administration increased serum IL-12 and IFN-gamma and induced tumor lymphocytic infiltration in all treated mice which was accompanied by tumor stabilization or regression in 40% of animals. The antitumor effect did not correlate with levels of IL-12 or IFN-gamma nor with the intensity of tumor mononuclear infiltration. However, tumors from non-responder mice showed more abundance of Foxp3+ regulatory T cells and higher expression of the immunosuppressive molecules PD-1, PD-L1, VEGF, CTLA-4, IDO, and IL-10 than those that responded to therapy. CONCLUSIONS: Although long-term induction of IL-12 expression in the liver can inhibit HCC growth, the efficacy of the treatment appears to be limited by the activation of immunosuppressive mechanisms. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Gene therapy | es_ES |
dc.subject | Interleukin-12 | es_ES |
dc.subject | Regulatory T cells | es_ES |
dc.subject | Foxp3 | es_ES |
dc.subject | Hepatocellular carcinoma | es_ES |
dc.subject | Tet-on system | es_ES |
dc.title | Induction of immunosuppressive molecules and regulatory T cells counteracts the antitumor effect of interleukin-12-based gene therapy in a transgenic mouse model of liver cancer | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0168827807005065 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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