Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Lopez-Andres, N. (Natalia) | - |
dc.creator | Iñigo, C. (Carmen) | - |
dc.creator | Gallego, I. (Idoia) | - |
dc.creator | Diez-Martinez, J. (Javier) | - |
dc.creator | Fortuño, M.A. (María Antonia) | - |
dc.date.accessioned | 2012-05-02T08:07:46Z | - |
dc.date.available | 2012-05-02T08:07:46Z | - |
dc.date.issued | 2008 | - |
dc.identifier.citation | Lopez-Andres N, Inigo C, Gallego I, Diez J, Fortuno MA. Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes. Endocrinology 2008 Oct;149(10):4970-4978. | es_ES |
dc.identifier.issn | 1945-7170 | - |
dc.identifier.uri | https://hdl.handle.net/10171/21853 | - |
dc.description.abstract | Aldosterone (ALDO) may induce cardiac hypertrophy by nonhemodynamic mechanisms that are not completely defined. Cardiotrophin-1 (CT-1) is a cytokine that exerts hypertrophic actions on isolated cardiomyocytes and promotes cardiac hypertrophy in vivo. We investigated whether ALDO induces CT-1 expression in HL-1 cardiomyocytes aiming at the possibility that the cytokine is involved in ALDO-induced cardiomyocyte hypertrophy. mRNA and protein expression were quantified by RT-PCR and Western blot. Cardiomyocyte area, as an index of hypertrophy, was assayed by image analysis in phalloidin-stained HL-1 cells. ALDO addition to adult HL-1 cardiomyocytes increased (P<0.01) CT-1 mRNA and protein expression in a concentration-dependent manner. This effect was abrogated by actinomycin D, the mineralocorticoid and glucocorticoid receptor antagonists spironolactone and RU486, respectively, and the p38 MAPK blocker SB203580. CT-1 signaling pathway blockade with specific antibodies against the cytokine and its two receptor subunits avoided (P<0.01) alpha-sarcomeric actin and c-fos protein overexpression as well as cell size increase induced by ALDO in HL-1 cells. In vivo, a single ALDO injection acutely increased (P<0.01) the myocardial expression of CT-1 in C57BJ6 wild-type mice but not CT-1-null mice. The bolus of the mineralocorticoid increased (P<0.01) ANP and c-fos mRNA expression in the myocardium of wild-type mice, whereas no changes were observed in CT-1-null mice. In summary, ALDO induces CT-1 expression in adult HL-1 cardiomyocytes via genomic and nongenomic mechanisms. CT-1 up-regulation could have relevance in the direct hypertrophic effects of ALDO in cardiomyocytes. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Endocrine Society | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Aldosterone/pharmacology | es_ES |
dc.subject | Cardiomegaly/physiopathology | es_ES |
dc.subject | Cytokines/genetics | es_ES |
dc.subject | Cytokines/genetics | es_ES |
dc.subject | Myocytes, Cardiac/physiology | es_ES |
dc.title | Aldosterone induces cardiotrophin-1 expression in HL-1 adult cardiomyocytes | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://endo.endojournals.org/content/149/10/4970 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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