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Campo DC | Valor | Lengua/Idioma |
---|---|---|
dc.creator | Gonzalez, A. (Arantxa) | - |
dc.creator | Ravassa, S. (Susana) | - |
dc.creator | Loperena, I. (Iñigo) | - |
dc.creator | Lopez-Salazar, M.B. (María Begoña) | - |
dc.creator | Beaumont, J. (Javier) | - |
dc.creator | Querejeta, R. (Ramón) | - |
dc.creator | Larman, M. (Mariano) | - |
dc.creator | Diez-Martinez, J. (Javier) | - |
dc.date.accessioned | 2012-05-02T11:24:19Z | - |
dc.date.available | 2012-05-02T11:24:19Z | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Gonzalez A, Ravassa S, Loperena I, Lopez B, Beaumont J, Querejeta R, et al. Association of depressed cardiac gp130-mediated antiapoptotic pathways with stimulated cardiomyocyte apoptosis in hypertensive patients with heart failure. J Hypertens 2007 Oct;25(10):2148-2157. | es_ES |
dc.identifier.issn | 1473-5598 | - |
dc.identifier.uri | https://hdl.handle.net/10171/21863 | - |
dc.description.abstract | OBJECTIVE: To investigate whether the glycoprotein (gp130)-mediated survival pathway, which protects cardiomyocytes from apoptosis, is depressed in left ventricular hypertrophy hypertensive patients with chronic heart failure. METHODS: Transvenous endomyocardial biopsies were obtained in 52 hypertensive patients with left ventricular hypertrophy: 28 without heart failure and 24 with heart failure. gp130 and gp130-dependent antiapoptotic pathways p42/44 mitogen-activated protein kinase (MAPK) and phosphatidylinositol-3 kinase (PI3K)/protein kinase B (Akt) as well as gp130 agonist cardiotrophin-1 were analyzed by reverse transcriptase-polymerase chain reaction and western blot. Apoptosis was assessed by DNA end-labeling (TUNEL), caspase-3 immunostaining and caspase substrate poly(ADP-ribose) polymerase cleavage. RESULTS: gp130 protein expression (P < 0.05) and p42/44 MAPK and PI3K/Akt activation (P < 0.01) were decreased in heart-failure hypertensive patients compared with nonheart-failure hypertensive individuals. No changes in gp130 mRNA expression were found between the two groups. Cardiotrophin-1 was increased (P < 0.05) at both the mRNA and protein levels in heart-failure hypertensive individuals compared with nonheart-failure hypertensive individuals. Cardiomyocyte apoptosis was increased (P < 0.01) in heart-failure hypertensive individuals compared with nonheart-failure hypertensive individuals. Inverse correlations (P < 0.01) occurred between cardiomyocyte apoptosis and p42/44 MAPK and PI3K/Akt activation in all hypertensive patients. Cardiotrophin-1 correlated inversely (r = -0.554, P < 0.05) with gp130 in all hypertensive individuals. In cultured HL-1 cardiomyocytes, cardiotrophin-1 decreased (P < 0.05) the gp130:phosphorylated gp130 (at Ser782) ratio and increased (P < 0.05) gp130ubiquitination. CONCLUSIONS: An association exists between depression of the gp130 cytoprotective pathway and stimulation of cardiomyocyte apoptosis in hypertensive patients that develop heart failure. Whether the excess of cardiotrophin-1 induces ligand-induced receptor down-regulation in these patients requires further study. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Lippincott, Williams & Wilkins | es_ES |
dc.rights | info:eu-repo/semantics/closedAccess | - |
dc.subject | Cytokine Receptor gp130/metabolism | es_ES |
dc.subject | Heart Failure/metabolism | es_ES |
dc.subject | Heart Failure/pathology | es_ES |
dc.subject | Hypertension/metabolism | es_ES |
dc.subject | Hypertension/pathology | es_ES |
dc.subject | Myocytes, Cardiac/metabolism | es_ES |
dc.subject | Myocytes, Cardiac/pathology | es_ES |
dc.title | Association of depressed cardiac gp130-mediated antiapoptotic pathways with stimulated cardiomyocyte apoptosis in hypertensive patients with heart failure | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://bit.ly/JN92Xe | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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