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Dadun > Depósito Académico > Facultad de Farmacia > Departamento de Ciencias de la Alimentación, Fisiología y Toxicología > DA - Farmacia - CAFT - Artículos de revista >

Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum
Autor(es) : Menendez-Carreño, M. (María)
Garcia-Herreros, C. (Cecilia)
Ansorena, D. (Diana)
Astiasarán, I. (Iciar)
Palabras clave : Serum
Sterols
COPs
Linearity
Recovery
Repeatability
Fecha incorporación: 2008
Editorial : Elsevier
Versión del editor: http://dx.doi.org/10.1016/j.jchromb.2008.01.036
ISSN: 1570-0232
Cita: Menendez-Carreno M, Garcia-Herreros C, Astiasaran I, Ansorena D. Validation of a gas chromatography-mass spectrometry method for the analysis of sterol oxidation products in serum. J Chromatogr B Analyt Technol Biomed Life Sci. 2008 Mar 15;864(1-2):61-68.
Resumen
A validated gas chromatography-mass spectrometry (GC-MS) detection method for the quantitative analysis of sterol oxidation products (SOPs) in serum is described. After a lipid extraction procedure with chloroform-methanol, a cold saponification and purification by solid phase extraction, oxysterols were derivatized to form trimethyl-sylil-ethers which were subjected to GC-MS analysis. Calibration curves for cholesterol oxidation products showed determination coefficient (R(2)) of 1.0, with low values for the coefficient of variation of the response factors (< 1%). Detection and quantification limits were below 5 ng/mL and 10 ng/mL, respectively. Recovery data were between 77.65% and 110.29% (CV < 10% for all compounds). Good results were obtained for within- and between-day repeatability, with values below 10%. In conclusion, the method performed is suitable for the determination and quantification of SOPs in serum.
Enlace permanente: http://hdl.handle.net/10171/22124
Aparece en las colecciones: DA - Farmacia - CAFT - Artículos de revista

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Fichero:  amended manuscript II maria validacion.pdf
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