Full metadata record
DC FieldValueLanguage
dc.creatorDominguez, M. (Miguel)-
dc.creatorMontes, R. (Ramón)-
dc.creatorParamo, J.A. (José Antonio)-
dc.creatorAngles-Cano, E. (Eduardo)-
dc.date.accessioned2012-05-28T14:56:54Z-
dc.date.available2012-05-28T14:56:54Z-
dc.date.issued2002-
dc.identifier.citationDominguez M, Montes R, Paramo JA, Angles-Cano E. Bivalency of plasminogen monoclonal antibodies is required for plasminogen bridging to fibrin and enhanced plasmin formation. Biochim Biophys Acta 2002 Jul 29;1598(1-2):165-176.es_ES
dc.identifier.issn1878-2434-
dc.identifier.urihttp://hdl.handle.net/10171/22241-
dc.description.abstractBinding of plasminogen to fibrin and cell surfaces is essential for fibrinolysis and pericellular proteolysis. We used surface plasmon resonance and enzyme kinetic analyses to study the effect of two mAbs (A10.2, CPL15) on plasminogen binding and activation at fibrin surfaces. A10.2 is directed against the lysine-binding site (LBS) of kringle 4, whereas CPL15 recognises a region in kringle 1 outside the LBS. In the presence of CPL15 and A10.2 mAbs, binding of plasminogen (K(d)=1.16+/-0.22 micromol/l) to fibrin was characterised by a mAb concentration-dependent bell-shaped isotherm. A progressive increase in the concentration of mAbs at the surface was also detected, and reached a plateau corresponding to the maximum of plasminogen bound. These data indicated that at low mAb concentration, bivalent plasminogen-mAb-plasminogen ternary complexes are formed, whereas at high mAb concentration, a progressive shift to monovalent plasminogen-mAb binary complexes is observed. Plasmin formation in the presence of mAbs followed a similar bell-shaped profile. Monovalent Fab fragments of mAb A10.2 showed no effect on the binding of plasminogen, confirming the notion that a bivalent mAb interaction is essential to increase plasminogen binding and activation at the surface of fibrin.es_ES
dc.language.isoenges_ES
dc.publisherElsevieres_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectmAb bivalencyes_ES
dc.subjectPlasminogen bindinges_ES
dc.subjectPlasminogen activationes_ES
dc.subjectBiosensor analysises_ES
dc.subjectFibrin surfacees_ES
dc.subjectKringle domaines_ES
dc.titleBivalency of plasminogen monoclonal antibodies is required for plasminogen bridging to fibrin and enhanced plasmin formationes_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://www.sciencedirect.com/science/article/pii/S0167483802003643es_ES
dc.type.driverinfo:eu-repo/semantics/articlees_ES

Files in This Item:
File: 
BiochemBiophysActa2002_1598165.pdf
Description: 
Size: 
260,95 kB
Format: 
Adobe PDF


Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.