Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Orbe, J. (Josune) | - |
dc.creator | Montes, R. (Ramón) | - |
dc.creator | Zabalegui, N. (Natalia) | - |
dc.creator | Perez-Ruiz, A. (Ana) | - |
dc.creator | Paramo, J.A. (José Antonio) | - |
dc.date.accessioned | 2012-05-28T15:42:53Z | - |
dc.date.available | 2012-05-28T15:42:53Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Orbe J, Montes R, Zabalegui N, Perez-Ruiz A, Paramo JA. Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells. Thromb Res 1999 May 1;94(3):137-145. | es_ES |
dc.identifier.issn | 1879-2472 | - |
dc.identifier.uri | https://hdl.handle.net/10171/22262 | - |
dc.description.abstract | Heparin and other antithrombotic drugs besides their anticoagulant action could have a profibrinolytic effect. We have analyzed the effect of unfractionated heparin (UFH) and hirudin on PAI-1 gene expression in human umbilical vein endothelial cells (HUVEC). Cells were stimulated with UFH (1 and 10 IU/ml) and hirudin (20 and 100 TIU/ml). Samples were obtained before and 2, 6, and 24 hours after stimulation. mRNA analysis was conducted by reverse transcription followed by polymerase chain reaction, and PAI-1 antigen was determined by ELISA. Addition of UFH (10 IU/ml) to HUVEC resulted in a decrease of PAI-1 mRNA at 6 hours (40% reduction) and 24 hours (60% reduction) and PAI-1 antigen. Hirudin, however, did not modify significantly the PAI-1 mRNA nor the inhibitor secretion. The addition of UFH (10 or 100 IU/ml) to endotoxin-stimulated HUVEC also reduced the increased PAI-1 mRNA and antigen secretion (45%), whereas no effect could be observed with hirudin. Our results suggest that UFH, but not hirudin, by reducing the endothelial expression of PAI-1 might have a profibrinolytic effect. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Heparin | es_ES |
dc.subject | mRNA | es_ES |
dc.subject | Hirudin | es_ES |
dc.subject | Endotoxin | es_ES |
dc.subject | PAI-1 | es_ES |
dc.subject | HUVEC | es_ES |
dc.title | Evidence that heparin but not hirudin reduces PAI-1 expression in cultured human endothelial cells | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.sciencedirect.com/science/article/pii/S0049384898002035 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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