Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Orbe, J. (Josune) | - |
dc.creator | Chorda, C. (Carlos) | - |
dc.creator | Montes, R. (Ramón) | - |
dc.creator | Paramo, J.A. (José Antonio) | - |
dc.date.accessioned | 2012-05-28T15:48:42Z | - |
dc.date.available | 2012-05-28T15:48:42Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Orbe J, Chorda C, Montes R, Paramo JA. Changes in the fibrinolytic components of cultured human umbilical vein endothelial cells induced by endotoxin, tumor necrosis factor-alpha and interleukin-1alpha. Haematologica 1999 Apr;84(4):306-311. | es_ES |
dc.identifier.issn | 1592-8721 | - |
dc.identifier.uri | https://hdl.handle.net/10171/22267 | - |
dc.description.abstract | BACKGROUND AND OBJECTIVE: Vascular fibrinolysis, a major natural defense mechanism against thrombosis, is a highly regulated process. The aim of this study was to evaluate the effect of endotoxin, tumor necrosis factor-alpha (TNFalpha) and interleukin-1alpha (IL-1alpha), on the fibrinolytic potential of cultured human umbilical vein endothelial cells (HUVEC). DESIGN AND METHODS: Samples of stimulated conditioned media were collected over a period of 24 hours to determine: plasminogen activator (PA) and plasminogen activator inhibitor (PAI) activity, PAI-1 mRNA, tissue-type plasminogen activator (t-PA) antigen and urokinase-type plasminogen activator (u-PA) antigen. RESULTS: Similar changes were observed after endotoxin and cytokine stimulation: there was a significant increase of PAI activity (p<0.01), starting at 6 hours, which remained 24 hours after stimulation. PAI-1 mRNA also showed an important rise with these agents, although cytokines induced an earlier and more intense inhibitor response (up to 6-fold increase). PA activity increased significantly at 6 hours (p<0.01) to drop at 24 hours and was mainly related to the presence of u-PA. INTERPRETATION AND CONCLUSIONS: We conclude that endotoxin,+TNFalpha and IL-1alpha induce profound alterations in the fibrinolytic potential of HUVEC, characterized by an initial rise of activators (u-PA) followed by a strong increase of PAI-1. These changes may be of pathophysiologic significance for thrombosis and inflammatory reactions. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Ferrata Storti Foundation | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Endothelium | es_ES |
dc.subject | Endotoxin | es_ES |
dc.subject | Cytokines | es_ES |
dc.subject | PAI | es_ES |
dc.subject | Plasminogen activators | es_ES |
dc.title | Changes in the fibrinolytic components of cultured human umbilical vein endothelial cells induced by endotoxin, tumor necrosis factor-alpha and interleukin-1alpha | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.haematologica.org/content/84/4/306 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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