Detection of anti-hepatitis C virus antibodies by ELISA using synthetic peptides

Berasain, C. (Carmen); Garcia-Granero, M. (Marta); Riezu-Boj, J.I. (José Ignacio); Civeira, M.P. (María Pilar); Prieto, J. (Jesús); Borras-Cuesta, F. (Francisco)

Authors:

Berasain, C. (Carmen)

Garcia-Granero, M. (Marta)

Riezu-Boj, J.I. (José Ignacio)

Civeira, M.P. (María Pilar)

Prieto, J. (Jesús)

Borras-Cuesta, F. (Francisco)

Keywords:

Hepatitis C
ELISA assay
Anti-HCV antibodies
Synthetic peptides

Issue Date:

1993

Publisher:

Elsevier

Publisher Version:

http://www.sciencedirect.com/science/article/pii/S0168827805800133

ISSN:

1600-0641

Citation:

Berasain C, Garcia-Granero M, Riezu-Boj JI, Civeira MP, Prieto J, Borras-Cuesta F. Detection of anti-hepatitis C virus antibodies by ELISA using synthetic peptides. J Hepatol 1993 Apr;18(1):80-84.

Abstract

A novel ELISA assay for the detection of anti-hepatitis C virus antibodies in the sera of infected individuals is described. This assay is based on a mixture of three 15-amino acid synthetic peptides encompassing regions of core and NS4 proteins of hepatitis C virus. Comparison with other available ELISA assays based on recombinant polypeptides shows that, short synthetic peptides have the advantage over some larger recombinant peptides by giving higher specificity without loss of sensitivity.

URI:

https://hdl.handle.net/10171/23060

Appears in Collections:

DA - CIMA - Hepatología - Oncobiología - Artículos de revista
DA - CUN - Medicina interna - Artículos de revista

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