Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Rodriguez-Madoz, J.R. (Juan Roberto) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.creator | Smerdou, C. (Cristian) | - |
dc.date.accessioned | 2012-09-25T10:42:47Z | - |
dc.date.available | 2012-09-25T10:42:47Z | - |
dc.date.issued | 2005 | - |
dc.identifier.citation | Rodriguez-Madoz JR, Prieto J, Smerdou C. Semliki forest virus vectors engineered to express higher IL-12 levels induce efficient elimination of murine colon adenocarcinomas. Mol Ther 2005 Jul;12(1):153-163. | es_ES |
dc.identifier.issn | 1525-0024 | - |
dc.identifier.uri | https://hdl.handle.net/10171/23177 | - |
dc.description.abstract | To evaluate the use of alphavirus vectors for tumor treatment we have constructed and compared two Semliki Forest virus (SFV) vectors expressing different levels of IL-12. SFV-IL-12 expresses both IL-12 subunits from a single subgenomic promoter, while in SFV-enhIL-12 each IL-12 subunit is expressed from an independent subgenomic promoter fused to the SFV capsid translation enhancer. This latter strategy provided an eightfold increase of IL-12 expression. We chose the poorly immunogenic MC38 colon adenocarcinoma model to evaluate the therapeutic potential of SFV vectors. A single intratumoral injection of 10(8) viral particles of SFV-IL-12 or SFV-enh-IL-12 induced>or=80% complete tumor regressions with long-term tumor-free survival. However, lower doses of SFV-enhIL-12 were more efficient than SFV-IL-12 in inducing antitumoral responses, indicating a positive correlation between the IL-12 expression level and the therapeutic effect. Moreover, repeated intratumoral injections of suboptimal doses of SFV-enhIL-12 increased the antitumoral response. In all cases SFV vectors were more efficient at eliminating tumors than a first-generation adenovirus vector expressing IL-12. In addition, the antitumoral effect of SFV vectors was only moderately affected by preimmunization of animals with high doses of SFV vectors. This antitumoral effect was produced, at least partially, by a potent CTL-mediated immune response. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Nature | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Semliki Forest virus vectors | es_ES |
dc.subject | SFV translation enhancer | es_ES |
dc.subject | Gene therapy | es_ES |
dc.subject | MC38 | es_ES |
dc.subject | Colon adenocarcinoma | es_ES |
dc.subject | IL-12 | es_ES |
dc.title | Semliki forest virus vectors engineered to express higher IL-12 levels induce efficient elimination of murine colon adenocarcinomas | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.nature.com/mt/journal/v12/n1/full/mt20051278a.html | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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