Full metadata record
DC Field | Value | Language |
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dc.creator | McKee, H.J. (Harley J.) | - |
dc.creator | T'sao, P.Y. (Patricia Y.) | - |
dc.creator | Vera, M. (María) | - |
dc.creator | Fortes, P. (Puri) | - |
dc.creator | Strayer, D.S. (David S.) | - |
dc.date.accessioned | 2012-09-28T08:46:07Z | - |
dc.date.available | 2012-09-28T08:46:07Z | - |
dc.date.issued | 2004 | - |
dc.identifier.citation | McKee HJ, T'sao PY, Vera M, Fortes P, Strayer DS. Durable cytotoxic immune responses against gp120 elicited by recombinant SV40 vectors encoding HIV-1 gp120 +/- IL-15. Genet Vaccines Ther 2004 Aug 23;2(1):10. | es_ES |
dc.identifier.issn | 1479-0556 | - |
dc.identifier.uri | https://hdl.handle.net/10171/23258 | - |
dc.description.abstract | BACKGROUND: A vaccine that elicits durable, powerful anti-HIV immunity remains an elusive goal. In these studies we tested whether multiple treatments with viral vector-delivered HIV envelope antigen (gp120), with and without IL-15, could help to approach that goal. For this purpose, we used recombinant Tag-deleted SV40-derived vectors (rSV40s), since they do not elicit neutralizing antibody responses, and so can be given multiply without loss of transduction efficiency. METHODS: SV(gp120) carried the coding sequences for HIV-1NL4-3 Env, and SV(mIL-15) carried the cDNA for mouse IL-15. Singly, and in combination, these two vectors were given monthly to BALB/cJ mice. Cytotoxic immunity and cytotoxic memory were tested in direct cytotoxicity assays using unselected effector cells. Antibody vs. gp120 was measured in a binding assay. In both cases, targets were P815 cells that were stably transfected with gp120. RESULTS: Multiple injections of SV(gp120) elicited powerful anti-gp120 cytolytic activity (>70% specific lysis) by unselected spleen cells. Cells from multiply-immunized mice that were rested 1 year after their last injections still showed >60% gp120-specific lysis. Anti-gp120 antibody was first detected after 2 monthly injections of SV(gp120) and remained elevated thereafter. Adding SV(mIL-15) to the immunization regimen dramatically accelerated the development of memory cytolytic responses, with >/= 50% specific lysis seen 1 month after two treatments. IL-15 did not alter the development of antibody responses. CONCLUSIONS: Thus, rSV40s encoding antigens and immunostimulatory cytokines may be useful tools for priming and/or boosting immune responses against HIV. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | BioMed Central | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | gp120 | es_ES |
dc.subject | recombinant SV40 vectors | es_ES |
dc.subject | HIV-1 gp120 +/- IL-15 | es_ES |
dc.subject | HIV-1 gp120 +/- IL-15 | es_ES |
dc.title | Durable cytotoxic immune responses against gp120 elicited by recombinant SV40 vectors encoding HIV-1 gp120 +/- IL-15 | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://www.gvt-journal.com/content/2/1/10 | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
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