Transformed but not normal hepatocytes express UCP2
Keywords: 
Uncoupling protein
Hepatoma
Mitochondrion
Tumor marker
Hypomethylation
Issue Date: 
1998
Publisher: 
Elsevier
ISSN: 
1873-3468
Citation: 
Carretero MV, Torres L, Latasa U, Garcia-Trevijano ER, Prieto J, Mato JM, et al. Transformed but not normal hepatocytes express UCP2. FEBS Lett 1998 Nov 13;439(1-2):55-58.
Abstract
Uncoupling protein 2 (UCP2) expression in liver is restricted to non-parenchymal cells. By means of differential display screening between normal rat liver and H4IIE hepatoma cells we have isolated a cDNA clone encompassing part of UCP2 cDNA. Northern blot analysis revealed that UCP2 is expressed in some hepatocarcinoma cell lines, while it is absent in adult hepatocytes. UCP2 mRNA in H4IIE cells was downregulated when cells were cultured for 36 h in 0.1% serum and its expression was restored upon addition of 10% serum or phorbol esters. Hypomethylation of UCP2 was observed in transformed UCP2 expressing cells. Our results indicate that UCP2 is expressed in some hepatocarcinoma cell lines and that serum components may participate in maintaining elevated UCP2 levels.

Files in This Item:
File: 
FEBS_Letters_1998_439_55.pdf
Description: 
Size: 
171,49 kB
Format: 
Adobe PDF


Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.