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Dadun > Depósito Académico > CIMA (Centro de Investigación Médica Aplicada) > Área de Terapia génica y Hepatología > Inmunología clínica > DA - CIMA - Terapia génica y Hepatología - Inmunología clínica - Artículos de revista >

The stilbene disulfonic acid DIDS stimulates the production of TNF-alpha in human lymphocytes
Autor(es) : Qian, C. (Cheng)
Diez, J. (Javier)
Larrea, E. (Esther)
Garciandia, A. (Ana)
Arrazola, A. (Arantxa)
Civeira, M.P. (María Pilar)
Medina, J.F. (Juan Francisco)
Prieto, J. (Jesús)
Palabras clave : 4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid/analogs & derivatives
Lymphocytes/drug effects
Tumor Necrosis Factor-alpha/biosynthesis
Fecha incorporación: 1992
Editorial : Elsevier
Versión del editor: http://www.sciencedirect.com/science/article/pii/0006291X9290210C
ISSN: 1090-2104
Cita: Qian C, Diez J, Larrea E, Garciandia A, Arrazola A, Civeira MP, et al. The stilbene disulfonic acid DIDS stimulates the production of TNF-alpha in human lymphocytes. Biochem Biophys Res Commun 1992 Dec 30;189(3):1268-1274.
Resumen
Exposure of human peripheral blood mononuclear cells (PBMC) to the stilbene derivative DIDS (4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid) (60 microM and above) significantly increased the release of tumor necrosis factor-alpha (TNF-alpha), as determined by TNF-alpha activity in the incubation media. When the TNF-alpha message was analyzed in PBMC by a reverse transcription/polymerase chain reaction (RT/PCR)-based procedure, it was found that incubation with DIDS (60 microM) was followed by a time-dependent accumulation of TNF-alpha mRNA. Measurements of intracellular pH showed that the presence of increasing concentrations of DIDS resulted in a progressive intracellular alkalinization of PBMC. It is suggested that the known DIDS effect of inhibiting transmembrane anion exchange, i.e., chloride/bicarbonate exchange, might play a role in the stimulation of TNF-alpha production by PBMC exposed to DIDS.
Enlace permanente: http://hdl.handle.net/10171/23321
Aparece en las colecciones: DA - CIMA - Terapia génica y Hepatología - Inmunología clínica - Artículos de revista

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Fichero:  BiochemBiophysResCommun1992_189_3.pdf
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