Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Larrea, E. (Esther) | - |
dc.creator | Alberdi, A. (A.) | - |
dc.creator | Castelruiz, Y. (Yurdana) | - |
dc.creator | Boya, P. (Patricia) | - |
dc.creator | Civeira, M.P. (María Pilar) | - |
dc.creator | Prieto, J. (Jesús) | - |
dc.date.accessioned | 2012-10-09T10:15:32Z | - |
dc.date.available | 2012-10-09T10:15:32Z | - |
dc.date.issued | 2001 | - |
dc.identifier.citation | Larrea E, Alberdi A, Castelruiz Y, Boya P, Civeira MP, Prieto J. Expression of interferon-alpha subtypes in peripheral mononuclear cells from patients with chronic hepatitis C: a role for interferon-alpha5. J Viral Hepat 2001 Mar;8(2):103-110. | es_ES |
dc.identifier.issn | 1365-2893 | - |
dc.identifier.uri | https://hdl.handle.net/10171/23330 | - |
dc.description.abstract | Interferon (IFN)-alpha is a family of antiviral proteins encoded by different genes. The biological significance of the existence of various IFN-alpha subtypes is not clear. We have investigated the interferon system in chronic hepatitis C virus (HCV) infection, a disease that responds to interferon-alpha2 therapy in only a limited proportion of cases. We analysed the expression of interferon regulatory factor (IRF)-1, IRF-2, and IFN-alpha subtypes in nonstimulated and Sendai virus-stimulated peripheral blood mononuclear cells (PBMC) from HCV infected patients and healthy controls. We observed that the IRF-1 mRNA and IRF-1/IRF-2 ratios were increased in PBMC from hepatitis C patients with respect to normal subjects. Sendai virus stimulation of PBMC led to a significant increase in the levels of IRF-1, IRF-2 and IFN-alpha mRNAs and in the production of IFN-alpha protein with respect to basal values in healthy controls as well as in patients with HCV infection. In addition, we found that while natural HCV infection induced increased IFN-alpha5 expression in PBMC, in vitro infection of these cells with Sendai virus caused a raise in the expression of IFN-alpha8 in both patients and normal controls. In summary, our results indicate that virus-induced activation of the IFN system in human PBMC is associated with selective expression of individual IFN-alpha subtypes, IFN-alpha5 being the specific subtype induced in PBMC from patients with chronic HCV infection. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Blackwell Publishing | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Hepatitis C virus | es_ES |
dc.subject | Interferon regulatory factor | es_ES |
dc.subject | Interferon-a subtypes | es_ES |
dc.subject | Peripheral blood mononuclear cells | es_ES |
dc.subject | Sendai virus | es_ES |
dc.title | Expression of interferon-alpha subtypes in peripheral mononuclear cells from patients with chronic hepatitis C: a role for interferon-alpha5 | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.relation.publisherversion | http://onlinelibrary.wiley.com/doi/10.1046/j.1365-2893.2001.00273.x/abstract | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
Files in This Item:
Statistics and impact
Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.