Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Diaz-Lagares, A. (Ángel) | - |
dc.creator | Alegre-Martinez, E. (Estibaliz) | - |
dc.creator | LeMaoult, J. (Joël) | - |
dc.creator | Carosella, E.D. (Edgardo D.) | - |
dc.creator | Gonzalez-Hernandez, A. (Alvaro) | - |
dc.date.accessioned | 2012-10-24T09:59:07Z | - |
dc.date.available | 2012-10-24T09:59:07Z | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Diaz-Lagares A, Alegre E, LeMaoult J, Carosella ED, Gonzalez A. Nitric oxide produces HLA-G nitration and induces metalloprotease-dependent shedding creating a tolerogenic milieu. Immunology 2009 Mar;126(3):436-445. | es_ES |
dc.identifier.issn | 0019-2805 | - |
dc.identifier.uri | https://hdl.handle.net/10171/23479 | - |
dc.description.abstract | Human leucocyte antigen G (HLA-G) is a tolerogenic molecule that protects the fetus from maternal immune attack, may favour tumoral immunoescape and is up-regulated in viral and inflammatory diseases. The aim of this work was to discover if nitric oxide (NO) could affect HLA-G expression or function because NO is an important modulator of innate and adaptive immunity. For this purpose HLA-G expression and function were analysed following treatment with a NO donor or a peroxynitrite donor in various cell lines expressing HLA-G either spontaneously or upon transfection. Results showed NO-dependent nitration of both cellular and soluble HLA-G protein, but not all HLA-G moieties underwent nitration. Endogenous biosynthesis of NO by both U-937-HLA-G1 and M8-HLA-G5 stable transfectants also caused HLA-G nitration. The NO decreased total HLA-G cellular protein content and expression on the cell surface, while increasing HLA-G shedding into the culture medium. This effect was post-transcriptional and the result of metalloprotease activity. By contrast, NO pretreatment did not affect HLA-G capability to suppress NK cytotoxicity and lymphocyte proliferation. Our studies show that NO regulates the availability of HLA-G molecules without modifying their biological activities. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Blackwell Publishing | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Cytotoxicity, Immunologic | es_ES |
dc.subject | HLA-G Antigens | es_ES |
dc.subject | Histocompatibility Antigens Class I/drug effects/metabolism | es_ES |
dc.title | Nitric oxide produces HLA-G nitration and induces metalloprotease-dependent shedding creating a tolerogenic milieu | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1111/j.1365-2567.2008.02911.x | es_ES |
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