Polymerase Chain Reaction/methods Polymorphism, Restriction Fragment Length Tumor Necrosis Factor-alpha/genetics
Patiño-García A, Sotillo-Piñeiro E, Modesto C, Sierrasesúmaga L. Screening of the human tumor necrosis factor-alpha (TNF-alpha) gene promoter polymorphisms by PCR-DGGE analysis. Mutat Res. 1999 Aug;406(2-4):121-5.
We have designed a new PCR-DGGE technique that enables detection of base changes in the TNF-alpha gene promoter. Screening of 130 samples from Spanish children has shown that this technique accurately detects the altered band patterns induced by the presence of the polymorphisms at positions -376, -308, -238 and -163 of the promoter sequence. Although further analysis are needed to fully characterise the alterations detected, we believe that this PCR-DGGE technique is a rapid and sensitive first approach to the genetic characterisation of the TNF-alpha promoter