Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Patiño-García, A. (Ana) | - |
dc.creator | Sotillo, E. (Elena) | - |
dc.creator | Modesto, C. (C.) | - |
dc.creator | Sierrasesumaga, L. (Luis) | - |
dc.date.accessioned | 2012-11-22T15:22:29Z | - |
dc.date.available | 2012-11-22T15:22:29Z | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Patiño-García A, Sotillo-Piñeiro E, Modesto C, Sierrasesúmaga L. Screening of the human tumor necrosis factor-alpha (TNF-alpha) gene promoter polymorphisms by PCR-DGGE analysis. Mutat Res. 1999 Aug;406(2-4):121-5. | es_ES |
dc.identifier.issn | 1383-5742 | - |
dc.identifier.uri | https://hdl.handle.net/10171/23775 | - |
dc.description.abstract | We have designed a new PCR-DGGE technique that enables detection of base changes in the TNF-alpha gene promoter. Screening of 130 samples from Spanish children has shown that this technique accurately detects the altered band patterns induced by the presence of the polymorphisms at positions -376, -308, -238 and -163 of the promoter sequence. Although further analysis are needed to fully characterise the alterations detected, we believe that this PCR-DGGE technique is a rapid and sensitive first approach to the genetic characterisation of the TNF-alpha promoter | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Elsevier | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Polymerase Chain Reaction/methods | es_ES |
dc.subject | Polymorphism, Restriction Fragment Length | es_ES |
dc.subject | Tumor Necrosis Factor-alpha/genetics | es_ES |
dc.title | Screening of the human tumor necrosis factor-alpha (TNF-α) gene promoter polymorphisms by PCR–DGGE analysis | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1016/S1383-5726(99)00004-7 | es_ES |
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