Redondo P, Gimenez de Azcarate A, Marques L, Garcia-Guzman M, Andreu E, Prosper F. Amniotic membrane as a scaffold for melanocyte transplantation in patients with stable vitiligo. Dermatol Res Pract 2011;2011:532139.
Vitiligo is an acquired skin disease that significantly impacts the quality of
life of patients. Medical treatment of vitiligo includes the use of melanocyte
transplant, but the results are variable. We have treated 4 patients with either
focal or generalized stable vitiligo using a graft of autologous melanocytes'
culture on a denuded amniotic membrane (AM). A culture biopsy was obtained in
every patient and grown in melanocytes' media for 10-14 days after which cells
were transferred to a denuded AM and transplanted into the achromic lesions.
Patients were followed for up to 6 months using clinical assessment of achromic
lesions. Treated areas ranged between 4 cm(2) and 210.6 cm(2). Response to
treatment was excellent in all patients with 90-95% repigmentation success rate.
Our results demonstrate that transplantation of autologous melanocytes cultured
on AM is a new, simple, and effective treatment for stable vitiligo.