Prevention of diet-induced obesity by apple polyphenols in Wistar rats through regulation of adipocyte gene expression and DNA methylation patterns
Palabras clave : 
Nutrigenomics
Lipolysis
High-fat sucrose diet
Epigenetics
Adiposity
Fecha de publicación: 
2013
Editorial : 
Wiley
ISSN: 
1613-4125
Cita: 
Boque N, de la Iglesia R, de la Garza AL, Milagro FI, Olivares M, Banuelos O, et al. Prevention of diet-induced obesity by apple polyphenols in Wistar rats through regulation of adipocyte gene expression and DNA methylation patterns. Mol Nutr Food Res 2013 Mar 25;57(8):1473–1478
Resumen
This study was conducted to determine the mechanisms implicated in the beneficial effects of apple polyphenols (APs) against diet-induced obesity in Wistar rats, described in a previous study from our group. Supplementation of high-fat sucrose diet with AP prevented adiposity increase by inhibition of adipocyte hypertrophy. Rats supplemented with AP exhibited improved glucose tolerance while adipocytes isolated from these rats showed an enhanced lipolytic response to isoproterenol. AP intake led to reduced Lep, Plin, and sterol regulatory element binding transcription factor 1 (Srebf1) mRNA levels and increased aquaporin 7 (Aqp7), adipocyte enhancer binding protein 1 (Aebp1), and peroxisome proliferator-activated receptor gamma co-activator 1 alpha (Ppargc1a) mRNA levels in epididymal adipocytes. In addition, we found different methylation patterns of Aqp7, Lep, Ppargc1a, and Srebf1 promoters in adipocytes from apple-supplemented rats compared to high-fat sucrose fed rats. The administration of AP protects against body weight gain and fat deposition and improves glucose tolerance in rats. We propose that AP exerts the antiobesity effects through the regulation of genes involved in adipogenesis, lipolysis, and fatty acid oxidation, in a process that could be mediated in part by epigenetic mechanisms.

Ficheros en este registro:
Fichero: 
Figure 1.pdf
Descripción: 
Tamaño: 
35,62 kB
Formato: 
Adobe PDF
Fichero: 
Manuscript.pdf
Descripción: 
Tamaño: 
139,99 kB
Formato: 
Adobe PDF
Fichero: 
Table 1.pdf
Descripción: 
Tamaño: 
15,23 kB
Formato: 
Adobe PDF
Fichero: 
Figure 2.pdf
Descripción: 
Tamaño: 
97,17 kB
Formato: 
Adobe PDF


Los ítems de Dadun están protegidos por copyright, con todos los derechos reservados, a menos que se indique lo contrario.