Full metadata record
DC Field | Value | Language |
---|---|---|
dc.creator | Zaccheus, M.V. (Mona V.) | - |
dc.creator | Ali, T. (Tara) | - |
dc.creator | Cloeckaert, A. (Axel) | - |
dc.creator | Zygmunt, M. (Michel) | - |
dc.creator | Weintraub, A. (Andrej) | - |
dc.creator | Iriarte, M. (Maite) | - |
dc.creator | Moriyon, I. (Ignacio) | - |
dc.creator | Widmalm, G. (Göran) | - |
dc.date.accessioned | 2013-06-14T12:13:21Z | - |
dc.date.available | 2013-06-14T12:13:21Z | - |
dc.date.issued | 2013 | - |
dc.identifier.citation | Zaccheus MV, Ali T, Cloeckaert A, Zygmunt MS, Weintraub A, Iriarte M, et al. The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar. PLoS One 2013;8(1):e53941. | es_ES |
dc.identifier.issn | 1932-6203 | - |
dc.identifier.uri | https://hdl.handle.net/10171/29372 | - |
dc.description.abstract | The brucellae are Gram-negative bacteria that cause an important zoonosis. Studies with the main Brucella species have shown that the O-antigens of the Brucella smooth lipopolysaccharide are α-(1 → 2) and α-(1 → 3)-linked N-formyl-perosamine polysaccharides that carry M, A and C (A = M, A>M and A<M) epitopes relevant in serodiagnosis and typing. We report that, in contrast to the B. suis biovar 1 O-antigen used as a reference or to all described Brucella O-antigens, B. suis biovar 2 O-antigen failed to bind monoclonal antibodies of C (A = M), C (M>A) and M specificities. However, the biovar 2 O-antigen bound monoclonal antibodies to the Brucella A epitope, and to the C/Y epitope shared by brucellae and Yersinia enterocolitica O:9, a bacterium that carries an N-formyl-perosamine O-antigen in exclusively α-(1 → 2)-linkages. By (13)C NMR spectroscopy, B. suis biovar 1 but not B. suis biovar 2 or Y. enterocolitica O:9 polysaccharide showed the signal characteristic of α-(1 → 3)-linked N-formyl-perosamine, indicating that biovar 2 may altogether lack this linkage. Taken together, the NMR spectroscopy and monoclonal antibody analyses strongly suggest a role for α-(1 → 3)-linked N-formyl-perosamine in the C (A = M) and C (M>A) epitopes. Moreover, they indicate that B. suis biovar 2 O-antigen lacks some lipopolysaccharide epitopes previously thought to be present in all smooth brucellae, thus representing a new brucella serovar that is M-negative, C-negative. Serologically and structurally this new serovar is more similar to Y. enterocolitica O:9 than to other brucellae. | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Public Library of Science | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.subject | Brucella | es_ES |
dc.subject | Gram negative bacteria | es_ES |
dc.subject | Lipopolysaccharides | es_ES |
dc.subject | Antigens | es_ES |
dc.subject | Monoclonal antibodies | es_ES |
dc.title | The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.type.driver | info:eu-repo/semantics/article | es_ES |
dc.identifier.doi | http://dx.doi.org/10.1371/journal.pone.0053941 | es_ES |
Files in This Item:
Statistics and impact
Items in Dadun are protected by copyright, with all rights reserved, unless otherwise indicated.