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dc.creatorZaccheus, M.V. (Mona V.)-
dc.creatorAli, T. (Tara)-
dc.creatorCloeckaert, A. (Axel)-
dc.creatorZygmunt, M. (Michel)-
dc.creatorWeintraub, A. (Andrej)-
dc.creatorIriarte, M. (Maite)-
dc.creatorMoriyon, I. (Ignacio)-
dc.creatorWidmalm, G. (Göran)-
dc.date.accessioned2013-06-14T12:13:21Z-
dc.date.available2013-06-14T12:13:21Z-
dc.date.issued2013-
dc.identifier.citationZaccheus MV, Ali T, Cloeckaert A, Zygmunt MS, Weintraub A, Iriarte M, et al. The epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovar. PLoS One 2013;8(1):e53941.es_ES
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10171/29372-
dc.description.abstractThe brucellae are Gram-negative bacteria that cause an important zoonosis. Studies with the main Brucella species have shown that the O-antigens of the Brucella smooth lipopolysaccharide are α-(1 → 2) and α-(1 → 3)-linked N-formyl-perosamine polysaccharides that carry M, A and C (A = M, A>M and A<M) epitopes relevant in serodiagnosis and typing. We report that, in contrast to the B. suis biovar 1 O-antigen used as a reference or to all described Brucella O-antigens, B. suis biovar 2 O-antigen failed to bind monoclonal antibodies of C (A = M), C (M>A) and M specificities. However, the biovar 2 O-antigen bound monoclonal antibodies to the Brucella A epitope, and to the C/Y epitope shared by brucellae and Yersinia enterocolitica O:9, a bacterium that carries an N-formyl-perosamine O-antigen in exclusively α-(1 → 2)-linkages. By (13)C NMR spectroscopy, B. suis biovar 1 but not B. suis biovar 2 or Y. enterocolitica O:9 polysaccharide showed the signal characteristic of α-(1 → 3)-linked N-formyl-perosamine, indicating that biovar 2 may altogether lack this linkage. Taken together, the NMR spectroscopy and monoclonal antibody analyses strongly suggest a role for α-(1 → 3)-linked N-formyl-perosamine in the C (A = M) and C (M>A) epitopes. Moreover, they indicate that B. suis biovar 2 O-antigen lacks some lipopolysaccharide epitopes previously thought to be present in all smooth brucellae, thus representing a new brucella serovar that is M-negative, C-negative. Serologically and structurally this new serovar is more similar to Y. enterocolitica O:9 than to other brucellae.es_ES
dc.language.isoenges_ES
dc.publisherPublic Library of Sciencees_ES
dc.rightsinfo:eu-repo/semantics/openAccesses_ES
dc.subjectBrucellaes_ES
dc.subjectGram negative bacteriaes_ES
dc.subjectLipopolysaccharideses_ES
dc.subjectAntigenses_ES
dc.subjectMonoclonal antibodieses_ES
dc.titleThe epitopic and structural characterization of Brucella suis biovar 2 O-polysaccharide demonstrates the existence of a new M-negative C-negative smooth Brucella serovares_ES
dc.typeinfo:eu-repo/semantics/articlees_ES
dc.relation.publisherversionhttp://dx.doi.org/10.1371/journal.pone.0053941es_ES
dc.type.driverinfo:eu-repo/semantics/articlees_ES

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